摘要
目的观察通脉方对精索静脉曲张(VC)模型大鼠精液质量及睾丸生精细胞热休克蛋白(HSP)60、HSP90表达的影响,并探讨其作用机制。方法将60只雄性SD大鼠随机分为空白对照组12只和模型组48只,模型组建立模型后随机分为模型对照组,通脉方低、中、高剂量组,每组12只。造模30天后开始给药,通脉方组分别给予浓度为0.545、1.089、2.178 g/mL的通脉方水煎液灌胃,其余2组给予生理盐水灌胃,干预48天后取左侧睾丸与附睾,采用HE染色观察大鼠睾丸病理变化,采用免疫组化法、Western Blot法检测睾丸组织HSP60、HSP90的表达,分析附睾精子浓度与精子总活力。结果模型对照组可见曲细精管排列紊乱,生精上皮细胞脱落变性,精子少见,通脉方各剂量组不同程度好转。与空白对照组比较,模型对照组HSP60、HSP90表达、精子浓度及精子总活力降低(P<0.05),与模型对照组比较,通脉方各剂量组HSP60、HSP90表达、精子浓度及精子总活力改善(P<0.05)。在HSP90表达方面通脉方呈现剂量依赖性。结论通脉方可上调VC模型大鼠HSP60、HSP90表达,抑制睾丸组织细胞凋亡,改善精液质量。
Objective To observe the effect of Tongmai Formula(TMF) on semen quality and expression of heat shock protein(HSP) 60,HSP90 in testicular spermatogenic cells of varicocele(VC) model rats.Methods Sixty male SD rats were randomly divided into blank control group(n=12) and model group(n=48).VC model were established in model group,and the 48 rats were randomly divided into model control group,TMF low,medium and high dose groups,12 in each group.Thirty days after establishing model,drugs were intervened for 48 days,TMF groups were gavaged with 0.545,1.089 and 2.178 g/mL of TMF decoction,respectively,and the other 2 groups were gavaged with normal saline.The left testis and epididymis were taken,the pathological changes of testis were observed by HE staining,the expressions of HSP60 and HSP90 in testis tissue were detected by immunohistochemistry and Western Blot.The concentration of epididymis sperm and total sperm motility were analyzed.Results In the model control group,there were disordered arrangement of seminiferous tubules,abscission and degeneration of spermatogenic epithelial cells,and rare sperms.In different TMF groups,the sperms were improved to varying degrees.Compared with the blank control group,the expressions of HSP60,HSP90,the sperm concentration and total sperm motility of the model control group decreased(P<0.05).Compared with the model control group,the expressions of HSP60,HSP90,the sperm concentration and total sperm motility of both TMF group improved(P<0.05),and showed dose-dependent phenomenon in expressions of HSP90.Conclusion TMF could up-regulate the expression of HSP60 and HSP90,inhibit testicular tissue cell apoptosis,and improves sperm quality in VC model rats.
作者
陈志威
赵家有
宋春生
CHEN Zhi-wei;ZHAO Jia-you;SONG Chunsheng(Graduate school of China Academy of Chinese Medical Sciences,Beijing,100700;China Press of Traditional Chinese Medicine Co.,Ltd,Beijing,100176)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2021年第7期855-859,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
北京市中医药科技发展资金项目(No.JJ2016-76)
中国科学技术协会青年人才托举工程项目(No.YESS20160138)。
