摘要
Photodynamic therapy(PDT)is a treatment that uses light-sensitive drugs and a light source to destroy cancer cells.Methylene blue(MB)is an efficient photosensitizing agent that has been widely used in PDT.However,MB suffers from the hypochromic effect that is caused by self-aggregation and DNA binding in vivo.It is also easily reduced to ineffective leucomethylene blue in the hypoxic environments surrounding solid tumors.In this work,we prepared MB carbon dots(MB-CDs)using MB as the carbon source.The MB-CDs not only inherit the PDT capabilities of MB,but also demonstrate good biocompatibility and low toxicity.Importantly,MB-CDs demonstrate excellent resistance to interference from reduction,aggregation,and DNA interactions.The MB-CDs exhibited satisfactory PDT activities both in vitro and in vivo.The tumor sizes were reduced to below 20%of their original volumes when irradiated by a 590-nm lightemitting diode source.
光动力疗法(PDT)是一种重要的癌症治疗手段,亚甲基蓝(MB)作为一种有效的光敏剂被广泛应用于光动力治疗.然而,MB在体内由于聚集以及与DNA结合而导致减色效应.同时,MB在实体瘤的低氧环境中容易被还原为无色的还原态leucoMB,从而丧失光动力能力.因此,我们以MB作为碳源,制备碳点MB-CDs.MBCDs不仅继承了MB的PDT能力,而且表现出良好的生物相容性和低毒性.重要的是,MB-CDs具有出色的抗还原、抗聚集和抗DNA干扰的能力,并且MB-CDs在体外和体内均表现出良好的PDT活性,仅用590 nm LED光源照射一次,即可使肿瘤体积减小到原始体积的20%以下.
基金
financially supported by the National Natural Science Foundation of China (NSFC, 21822804,21676047, 21421005, 21878039, and 21925802)
NSFC-Liaoning United Fund (U1908202 and U1608222)
