摘要
目的建立阴虚证动物模型,构建阴虚证动物模型系统性评价方法。方法 C57BLKS/J小鼠随机分为正常组和阴虚证组,每组9只。阴虚证组每日灌服浓度为1g/ml的伤阴药0.1ml/10g,连续4周;正常组灌服等体积超纯水。采用旷场实验观察小鼠中央格穿格次数、中央格停留时间百分比、旷场区运动总距离和速度;动态监测小鼠体温变化,并比较两组小鼠饮水量、唾液流率、粪便含水量、皮肤含水量、体质量、尿胆素原和尿胆红素、摄食量及拉力变化。结果旷场实验中阴虚证组小鼠中央格穿格次数、中央格停留时间百分比、旷场区运动总距离和速度均明显高于正常组(P<0.05或P<0.01)。灌服伤阴药4周后,与本组8:30比较,阴虚证组15:00左上肢和右下肢爪心温度升高,20:00四肢爪心温度均升高(P<0.05或P<0.01)。与正常组比较,第4周阴虚证组小鼠唾液流率、四肢皮肤含水量、体质量及食量均下降,四肢爪心温度、饮水量均增加(P<0.05或P<0.01),尿胆素原弱阳性率、尿胆红素阳性率升高(P<0.01)。结论耗伤阴液法能够成功建立阴虚证动物模型;构建了基于"五心烦热、潮热、口干、皮肤干燥、形体消瘦、尿赤"的阴虚证典型表现的评价方法。
Objective To establish an animal model of yin-deficiency syndrome and to establish a systematic evaluation method of yin-deficiency syndrome. Methods C57 BLKS/J mice were randomly divided into normal group and yin-deficiency syndrome group, with nine rats in each group. Yin-deficiency syndrome group was administrated with 0.1 ml/10 g body weight yin-damaging drugs at a concentration of 1 g/ml daily for 4 weeks, and the normal group was given the same volume of ultra-pure water. Open field test was carried out to assess the central grid-crossing times, the percentage of the central grid-staying time, the total distance and speed of movement in the open field. The body temperature of mice was dynamically monitored, and the changes of water intake, saliva flow rate, fecal water content, skin water content, body mass, urobilinogen and urinary bilirubin, food intake and tensile force were measured and compared between groups. Results In the open field test, the central grid-crossing times, the percentage of the central grid-staying time, the total distance and speed of movement in the open field were significantly higher in the yin-deficiency syndrome group than those in the normal group(P<0.05 or P<0.01). After 4 weeks of administration of yin-damaging drugs, it was found that the claw center temperature of the upper left limb and the lower right limb increased from 8:30 a.m. to 15:00 p.m., and all four limbs had higher temperature at 20:00 p.m. compared to those at 8:30 a.m.(P<0.05 or P<0.01). Compared to the normal group, the yin-deficiency syndrome group had lower saliva flow rate, skin water content of the limbs, body mass and food intake, higher paw temperature of the four limbs and water intake(P<0.05 or P<0.01), as well as higher weak-positive rate of urobilinogen and positive rate of urinary bilirubin at the 4 th week(P<0.01). Conclusion The method of consuming yin-fluid can be used to successfully establish an animal model of yin-deficiency syndrome. The evaluation method based on the typical manifestations
作者
孙晓霞
任威铭
崔荣兴
陈宁
周雯
战丽彬
SUN Xiaoxia;REN Weiming;CUI Rongxing;CHEN Ning;ZHOU Wen;ZHAN Libin(School of Traditional Chinese Medicine&School of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing,210023)
出处
《中医杂志》
CSCD
北大核心
2021年第13期1156-1163,共8页
Journal of Traditional Chinese Medicine
基金
国家重点研发计划(2018YFC1704400)
江苏省研究生科研创新计划(KYCX211700)。
关键词
阴虚证
证候模型
模型评价
yin-deficiency syndrome
syndrome model
model evaluation
