摘要
沃尔巴克氏体(Wolbachia)作为最成功的胞内共生菌之一,它能感染很多节肢动物与线虫,并影响宿主的繁殖来增加其自身的传播。目前,有关Wolbachia与宿主相互作用的研究主要集中在其对宿主生殖功能的调控上。除了生殖系统外,Wolbachia也能感染宿主的体细胞,对宿主嗅觉、取食、学习与记忆、睡眠等多方面行为产生影响。然而,Wolbachia对宿主取食节律的影响还未见报道。本研究通过透射电镜和荧光原位杂交实验发现,wMel Wolbachia广泛分布于黑腹果蝇(Drosophila melanogaster)的大脑中。同时,我们利用Multibeam(MB5-MB)果蝇活性监测器检测果蝇取食节律。结果发现,不论是感染Wolbachia,还是未感染Wolbachia对照组的果蝇,雌性果蝇比雄性果蝇出现在靠近食物端的次数更多,即取食偏好更高;感染Wolbachia的雌性果蝇,与对照组相比,在夜晚(ZT19-23)的取食偏好更高;相反的,感染Wolbachia的雄性果蝇,与对照组相比,在下午至夜晚(ZT15-23)的取食偏好更低,而在上午(ZT1-9)的取食偏好更高,表明Wolbachia感染对果蝇的取食节律有影响,本文为Wolbachia对无脊椎动物宿主行为影响的研究提供一定参考价值。
Wolbachia are genus of bacteria found within the tissues of several groups of arthropods and nematode, they can control the host’s reproductive behavior to increase its own spread. At present, the research on the interaction between Wolbachia and the host is mainly focused on the regulation of the host’s reproduction. In addition to the reproductive system, Wolbachia can also infect the host’s somatic cells and affect the host’s sense of olfactory, feeding, learning and memory, sleep and many other behaviors. However, the influence of Wolbachia on the host’s feeding rhythm has not been reported yet.In this study, we found that wMel Wolbachia is widely distributed in the brain of Drosophila melanogaster through transmission electron microscopy and fluorescence in situ hybridization. At the same time, we used Multibeam(MB5-MB) Drosophila Activity Monitor System to detect the feeding rhythm of them.The results showed that whether the Wolbachia infected flies or uninfected flies, female flies appeared near the food side more frequently than male flies, that is, showed a higher feeding preference;Wolbachia infected female flies, compared with the uninfected female flies, the feeding preference was higher at night(ZT19-23);on the contrary, Wolbachia infected male flies had lower feeding preference from afternoon to night(ZT15-23) compared with the control group, while the feeding preference was higher in the morning(ZT1-9).It shows that Wolbachia has an effect on the feeding rhythm of Drosophila. This study provides reference for the study of the influence of Wolbachia on the behavior of invertebrate hosts.
作者
文明明
赵治恒
毕洁
贺艳萍
舒在习
肖安红
WEN Ming-ming;ZHAO Zhi-heng;BI Jie;HE Yan-ping;SHU Zai-xi;XIAO An-hong(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan,430023,China)
出处
《武汉轻工大学学报》
2021年第3期29-34,共6页
Journal of Wuhan Polytechnic University
基金
武汉轻工大学大宗粮油精深加工教育部重点实验室开放课题(2019GYBQGDKFB07,2020JYBQGDKFA01)。
