摘要
以副溶血弧菌、创伤弧菌为指示菌,采用牛津杯法测定37株海洋放线菌菌株无菌发酵液的抑菌作用,筛选出抑菌作用较强的菌株并测定其抑菌谱;将抑菌作用较强的菌株接种到氨氮质量浓度为50 mg/L的培养基中发酵7 d,采用靛酚蓝分光光度法测定不同菌株发酵液中的氨氮质量浓度,筛选出兼具抑菌和降解氨氮功能的放线菌优良菌株;通过形态学观察、生理生化试验和16S rDNA序列分析,对筛选出的优良菌株进行种类鉴定。结果表明,15株放线菌对副溶血弧菌和创伤弧菌均具有抑制作用,其中菌株F28的抑菌作用最强,对副溶血弧菌和创伤弧菌的抑菌圈直径分别为(31.57±0.63)mm和(18.93±0.92)mm,且该菌株对供试的10种病原菌均有较强的抑制作用,抑菌谱较广;菌株F28具有氨氮降解能力,降解率为46.86%;结合抑菌作用测定结果,认为菌株F28兼具抑菌和降解氨氮功能,具有潜在的开发应用前景。初步鉴定F28菌株为梅久兰链霉菌。
Using Vibrio parahemolyticus and V.vulculus as indicator bacteria,the bacteriostatic activity of 37 strains of marine actinomyces in sterile fermentation broth was determined by Oxford cup method,and the bacteriostatic activity of the strains was screened out and their bacteriostatic spectra were determined.The strains with strong bacteriostatic activity were inoculated into the culture medium with a mass concentration of ammonia nitrogen of 50 mg/L and fermented for 7 d.The concentration of ammonia nitrogen in the fermentation broth of different strains was determined by indophenol blue spectrophotometry,and the excellent strains of actinomycetes with functions of bacteriostatic and degrading ammonia nitrogen were screened out.The selected strains were identified through morphological observation,physiological and biochemical test and 16S rDNA sequence analysis.The results showed that 15 strains of actinomycetes had inhibitory effects on both V.parahaemolyticus and V.vulnificus,in which strain F28 had the strongest inhibitory effect on V.parahaemolyticus and V.vulnificus,with diameter of the inhibitory ring of(31.57±0.63)mm and(18.93±0.92)mm,respectively.In addition,the strain had a strong inhibitory effect on all 10 pathogenic bacteria tested,with a wide inhibitory spectrum.Strain F28 had strong ammonia nitrogen degradation ability with degradation rate of 46.86%.Combined with the results of bacteriostatic activity,it was concluded that strain F28 as Streptomyces mediolani had both bacteriostatic and ammonia-nitrogen degradation functions,and potential development and application prospects.
作者
汤曼利
李舒馨
彭云
陈莹莹
王亚楠
马桂珍
暴增海
TANG Manli;LI Shuxin;PENG Yun;CHEN Yingying;WANG Yanan;MA Guizhen;BAO Zenghai(College of Marine Life and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China;Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China)
出处
《水产科学》
CAS
CSCD
北大核心
2021年第4期610-617,共8页
Fisheries Science
基金
江苏省农业科技自主创新资金资助项目(CX(19)3107)
江苏省研究生科研创新项目(KYCX20_2878).
关键词
海洋放线菌
弧菌
抑菌作用
氨氮降解
marine actinomycete
Vibrio
bacteriostatic effect
ammonia nitrogen degradation
