摘要
为寻找水稻白叶枯病天然生防药剂,本研究利用共培养和牛津杯法分离筛选植物根际土壤放线菌,共分离纯化获得65株放线菌菌株。在8株能够拮抗水稻白叶枯病菌Xanthomonas oryzae pv.oryzae(Xoo)放线菌菌株中,Sr-63菌株对水稻白叶枯病菌拮抗能力最强,其发酵液抑菌圈直径为46.4 mm±2.3 mm。优化发酵条件后,Sr-63菌株发酵液抑菌圈直径可达50.0 mm±1.2 mm;依据形态特征、生理生化特征和系统发育分析,鉴定菌株Sr-63为玫瑰轮丝链霉菌(Streptomyces roseoverticillatus)。抗菌谱实验结果表明,Sr-63菌株发酵液对水稻细菌性条斑病菌、大豆细菌性斑疹病菌、番茄细菌性叶斑病菌以及大豆细菌性斑点病菌4种植物病原细菌也有较好的拮抗作用。水稻白叶枯病防效实验结果表明,Sr-63菌株发酵液对水稻白叶枯病具有良好的防治效果,对‘Chai Nat 2’、‘KDML105’、‘Khao luang’3个水稻品种的病斑抑制率均达到了90%以上,且Sr-63菌株的发酵液防治效果优于化学农药叶枯唑。
In order to find natural biocontrol agents for bacterial leaf blight(BLB),the plant rhizosphere Actinomyces strains were isolated and screened by co-cultivation and Oxford cup method.A total of 65 rhizospheric Actinomyces strains were isolated,and 8 Actinomyces strains were screened against Xanthomonas oryzae pv.oryzae(Xoo),and the Actinomyces Sr-63 showed the strongest antibacterial activity against Xoo(inhibition zone diameter 46.4 mm±2.3 mm).After the optimization of fermentation conditions,the inhibition zone diameter could reach 50.0 mm±1.2 mm;The Actinomyces Sr-63 was identified as Streptomyces roseoverticillatus based on the morphological and phylogenetic analyses.The antibacterial spectrum experiment results indicated that Streptomyces roseoverticillatus Sr-63 also showed biological activities against 4 kinds of plant pathogenic bacteria,such as Xanthomonas oryzae pv.oryzicola,Xanthomonas campestris pv.glycines,Xanthomonas campestris pv.vesicatoria and Pseudomonas syringae pv.glycinea.In addition,the bio-control experiment showed that the fermentation broth of Streptomyces roseoverticillatus Sr-63 had a remarkably good prevention of rice bacterial blight disease,and the lesion inhibition rate of‘Chai Nat 2’,‘Khao luang’,‘KDML105’rice reached more than 90%,which was better than the control effect of bismerthiazol.
作者
史婷婷
马静静
郭鑫
倪峰
蒋冬花
SHI Ting-ting;MA Jing-jing;GUO Xin;NI Feng;JIANG Dong-hua(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2021年第3期403-412,共10页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(31570013)
浙江省基础公益研究计划资助项目(LGN18C010002)。
