摘要
目的建立苗药伤科止痛液微生物限度检查方法的适用性试验。方法参照《中国药典》四部,采用平皿法、薄膜过滤法进行方法学研究。结果三批次样品采用稀释法和薄膜过滤法计数回收比值均在0.5~2范围内;消除该品种的抑菌活性采用薄膜过滤法更合适。铜绿假单胞菌试验组常规法和稀释法均检出试验菌,金黄色葡萄球菌稀释法试验组检出试验菌。结论苗药伤科止痛液微生物计数检查采用薄膜过滤法,常规法用于其控制菌铜绿假单胞菌检查,稀释法用于其控制菌金黄色葡萄球菌检查。
OBJECTIVE To establish the applicability test of microbial limit test method for ShangKe ZhiTong liquid.METHODS According to the four parts of“Chinese Pharmacopoeia”,the dish method and membrane filtration method was used to conduct methodological research.RESULTS The three batches of samples were counted by the dilution method and the membrane filtration method and the recovery ratio was in the range of 0.5 to 2;to eliminate the bacteriostatic activity of this species,membrane filtration method was more suitable.The membrane filtration method was more suitable for eliminating the bacteriostatic activity of this variety.Both the conventional method and the dilution method were detected in the Pseudomonas aeruginosa test group,and the test bacteria were detected in the S.aureus dilution method test group.CONCLUSION The microbial count test of the ShangKe ZhiTong liquid of the Miao Medicine and uses the membrane filtration method,the conventional method is used for the control bacteria Pseudomonas aeruginosa,and the dilution method is used for the control bacteria Staphylococcus aureus.
作者
杨仁惠
陈永敏
罗玉玲
姚亚丽
杨元凤
YANG Ren-hui;CHEN Yong-min;LUO Yu-ling;YAO Ya-li;YANG Yuan-feng(The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550003,China)
出处
《海峡药学》
2021年第6期44-47,共4页
Strait Pharmaceutical Journal
基金
贵州省中医药管理局中医药、民族医药科学技术课题研究资助项目(QZYY2013-74)
贵州省国内一流建设学科建设项目:(中药学)(GNYL〔2017〕008号)-子课题项目:GNYL〔2017〕008号-7-号。
