摘要
目的:探讨亚抑菌浓度亚胺培南对多重耐药鲍曼不动杆菌生物膜的影响。方法:采用微量肉汤稀释法,测定医院2019年2月至2020年9月临床分离的36株亚胺培南耐药多重耐药鲍曼不动杆菌(MDR-AB)的最低抑菌浓度(MIC),结晶紫半定量染色法测定菌株生物膜形成水平。采用96孔聚苯乙烯板体外构建鲍曼不动杆菌生物膜的模型,用亚抑菌浓度亚胺培南作用于成熟生物膜。实验组Ⅰ、Ⅱ、Ⅲ组中分别用MIC、1/2MIC、1/4MIC的亚胺培南作用于已形成的成熟生物膜;对照组Ⅳ组不加亚胺培南。通过结晶紫半定量染色法对生物膜进行染色,共聚焦倒置显微镜下观察生物膜染色情况,采用多功能酶标仪在570 nm波长处测得生物膜OD值。结果:亚胺培南在实验组Ⅰ、Ⅱ、Ⅲ组中,即MIC、1/2MIC、1/4MIC浓度下作用于已成熟生物膜48、96 h后,所测得OD值与对照组Ⅳ组OD值之间进行比较,有显著性差异(P<0.05)。结论:亚抑菌浓度亚胺培南具有破坏成熟生物膜的能力,且随着浓度的升高,其破坏能力更强。
Objective:To investigate the influnce of Imipenem in Sub-inhibitory concentration against the biofilm formation of Multiple drug resistant Acinetobacter Bauman(MDR-Ab).Methods:The minimum inhibitory concentration(MIC)of imipenem against 36 imipenem-resistant multidrug-resistant Acinetobacter baumannii(MDR-Ab)strains isolated from a third-class A hospital from February 2019 to September 2020 was determined by microbroth dilution method.The biofilm formation level of the strains was determined by semi-quantitative crystal purple method.The biofilm model of Acinetobacter baumannii was constructed by 96-well polystyrene plate in vitro.After the mature biofilm was formed,imipenem at subinhibitory concentration was applied to the mature biofilm.The mature biofilms in experimental groupsⅠ,ⅡandⅢwere treated with imipenem of MIC,1/2MIC and 1/4MIC,respectively.Control groupⅣdid not receive imipenem.The biofilm was stained by crystalline violet semi-quantitative staining,and the biofilm staining was observed under a confocal inverted microscope.The OD value of the biofilm was measured at 570 nm by a multifunctional enzyme plate analyzer.Results:In experimental groupsⅠ,ⅡandⅢ,i.e.MIC,1/2MIC and 1/4MIC,imipenem was applied to the mature biofilm for 48h and 96h,the measured OD values were compared with the control group,and there were significant differences(P<0.05).Conclusion:The subinhibitory concentration of imipenem has the ability to destroy mature biofilm,and the damage ability becomes stronger with the increase of the concentration.
作者
方甜甜
宋沧桑
刘德华
唐树荣
徐磊
李兴德
杨焕芝
丁逸欣
任宝军
FANG Tian-tian;SONG Cang-sang;LIU De-hua;TANG Shu-rong;XU Lei;LI Xing-de;YANG Huan-zhi;DING Yi-xin;REN Bao-jun(Department of Pharmacy,Kuming Municipal First People′s Hospital,Yunnan Kunming 650031,China;Clinical Laboratory Kunming First People′s Hospital,Yunnan Kunming 650031,China;Dali University,Yunnan Dali 671000,China;Kuming Medical University,Yunnan Kunming 650500,China)
出处
《中国药物评价》
2021年第3期224-228,共5页
Chinese Journal of Drug Evaluation
基金
昆明市科技局项目(No.2019KJJH073)
云南省教育厅科学研究基金项目(No.2019J1295)
昆明市卫生科技人才培养项目[项2020-SW(后备)-36]。
关键词
多重耐药鲍曼不动杆菌
生物膜
亚抑菌浓度
亚胺培南
Multidrug-resistant Acinetobacter baumannii
Biofilm
Subinhibitory concentration
Imipenem
