摘要
目的分析耐碳青霉烯类鲍曼不动杆菌(CRAB)A、B、C 3个流行克隆(多携带bla OXA-23、bla OXA-51基因)同源性,测定其生物被膜形成能力,探讨该特性与碳青霉烯类耐药的相关性,了解3个克隆医院内流行原因。方法选取重庆医科大学附属第一医院CRAB A、B、C 3个流行克隆和碳青霉烯类敏感鲍曼不动杆菌(CSAB)临床分离株共87株,多位点序列分型(MLST)分析其同源性,结晶紫染色法定量分析其生物被膜形成能力。结果 A克隆为ST238相似型,B、C克隆均为ST238型;A、B、C流行克隆及CSAB组D值分别为0.325±0.145、0.811±0.295、0.306±0.133、0.913±0.626,该院CRAB流行克隆总体上生物被膜形成能力较CSAB组下降(0.470±0.301对0.913±0.626,P<0.05);各流行克隆间生物被膜形成能力存在差异,A、C型克隆间差异无统计学意义(P=0.432),均弱于B型克隆(P均<0.001);生物被膜形成能力与bla OXA-23、bla OXA-51基因无明确相关性(P均>0.05)。结论该院CRAB A、B、C流行克隆具有相同的起源,首次报道ST238及其相似型的医院内广泛流行;流行克隆生物被膜形成能力与碳青霉烯类耐药性的获得呈负相关性,提示克隆流行主要与耐药性获得及抗菌药物的压力选择相关;生物被膜形成能力在各克隆间存在差异,需警惕B型克隆的广泛传播。
Objective To analyze the homology,biofilm-forming ability,and risk factors of prevalent A,B,C clones(carrying blaOXA-23 and blaOXA-51 genes mostly)of carbapenem-resistant Acinetobacter baumannii(CRAB)relative to carbapenemsusceptible Acinetobacter baumannii(CSAB).Methods A total of 87 prevalent A,B,C clones of CRAB and CSAB strains were collected from the First Affiliated Hospital of Chongqing Medical University.Multilocus sequence typing(MLST)was used for homologyanalysis of clone A,B,C strains.Biofilm-forming ability of CRAB and CSAB was measured quantitatively via crystal violet staining.Results Clone A was measured to be homologous type of ST238,while clones B and C were ST238 type.In general,CRAB prevalent clones showed weaker biofilm-forming ability than CSAB strains(0.470±0.301 versus 0.913±0.626,P<0.05).CRAB clones A,B,and C varied in ability of biofilm formation.Clone A had comparative biofilm-forming ability to clone C(P=0.432).Both clone A and C were weaker than clone B in biofilm-forming ability(both P<0.001).Biofilm-forming ability was not associated with blaOXA-23 or blaOXA-51 genes in CRAB strains(both P>0.05).Conclusions Prevalent CRAB clone A,B,C are derived from the same origin.We are the first to report the prevalence of ST238 and the homologous types in a hospital.Biofilm-forming ability is negatively correlated with carbapenem resistance of Acinetobacter baumannii,which suggests that clone prevalence is mainly related to antibiotic resistance acquisition and antibiotic selective pressure.Biofilm-forming ability varies with the prevalent CRAB clone.The wide spread of clone B is of concern.
作者
尹珍
黄文祥
杨均均
李佳俊
刘成伟
YIN Zhen;HUANG Wenxiang;YANG Junjun;LI Jiajun;LIU Chengwei(Department of Infectious Diseases,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)
出处
《中国感染与化疗杂志》
CAS
CSCD
北大核心
2018年第2期184-189,共6页
Chinese Journal of Infection and Chemotherapy
基金
重庆市自然科学基金(CSTC2008BB5393)
重庆市教育委员会科技项目(KJ100311)
