摘要
本实验的目的是研究复方小钻方3种颗粒剂对慢性盆腔炎大鼠的作用及机制.除假手术组大鼠外,其余大鼠左子宫内机械损伤加子宫内注射细菌混合液(大肠杆菌加金黄色葡萄球菌),每只0.1 mL复制慢性盆腔炎模型.10 d后,取72只成功模型大鼠随机分为9组,包括模型组、左氧氟沙星组(42 mg/kg)、花红片组(870 mg/kg)及复方小钻方3种颗粒剂(分别为Ⅰ、Ⅱ和Ⅲ,每种2组,剂量均为9 g/kg和18 g/kg),另假手术处理的8只大鼠编入假手术组.假手术组和模型组大鼠灌胃0.5%羧甲基纤维素钠,其余大鼠按组别(10 mL/kg)灌胃不同浓度的左氧氟沙星或花红片或复方小钻方3种颗粒剂.连续灌胃28 d,记录体质量、子宫肿胀度和肿胀率,检测血清白介素-1β(IL-1β)、IL-6、丙二醛(MDA)和超氧化物歧化酶(SOD)水平.结果显示,与模型组比较,复方小钻方3种颗粒剂均可显著增加体质量、血清SOD水平(P<0.05或P<0.01),显著降低子宫肿胀度和肿胀率,血液白细胞总数和中心粒细胞比例,血清IL-1β、IL-6和丙二醛水平.复方小钻方3种颗粒剂均对慢性盆腔炎有良好的治疗效果,其机制可能与抑制促炎因子表达,增强机体抗氧化能力有关.其中复方小钻方效果最好的是颗粒Ⅱ.
To investigate the effect of three types of Xiaozuan Granules on chronic pelvic inflammation rats and its mechanisms.All the rats except the sham operation group were treated by injecting bacteria mixture(Escherichia coli and Staphylococcus aureus),0.1 mL per rat into the left uterus and mechanical injury to built the chronic pelvic inflammation models.Ten days later,seventy-two successful model rat were randomly divided into nine groups,including model group,Levofloxacin group(42 mg/kg),Huahong Pills group(870 mg/kg)and three types of Xiaozuan Granules groups(Ⅰ,ⅡandⅢ,one type two groups with the dosage 9 g/kg and 18 g/kg),another eight rats with sham operation were assigned into sham operation group.Rats in sham operation group and model group were received 10 mL/kg of 0.5%carboxymethylcellulose sodium,and rats in other groups were received different concentrations(10 mL/kg)of Levofloxacin,Huahong Pills or three types of Xiaozuan Granules,all the rats were treated by gastric intubation for twenty-eight consecutive days.The body mass,blood routine,uterus swelling degree and swelling ratio were recorded,and serum levels of Interleukin-1β(IL-1β),IL-6,Malondialdehyde(MDA)and Superoxide dismutase(SOD)were analyzed.As compared with model group,three types of Xiaozuan Granules groups could significantly increase the body mass,serum levels of SOD(P<0.05 or P<0.01),and significantly decrease uterus swelling degree and swelling ratio,levels of White Blood Cell(WBC),Neutrophilicgranulocyte(NEUT),IL-1β,IL-6 and MDA(P<0.05 or P<0.01).Three types of Xiaozuan Granules had good therapeutic effect on chronic pelvic inflammation,and may be related to inhibiting the expression of pro-inflammatory cytokine,enhancing the body-antioxidant injury ability.TypeⅡof Xiaozuan Granules was proved to be the best.
作者
陈柯贝
黄小妹
莫桂梅
曾诗颖
吴玉强
CHEN Ke-bei;HUANG Xiao-mei;MO Gui-mei;ZENG Shi-ying;WU Yu-qiang(School of Pharmaceutical Science, Guangxi University of Chinese Medicine, Nanning 530200, China;Pharmaceutical Factory, Guangxi University of Chinese Medicine, Nanning 530023, China)
出处
《陕西科技大学学报》
北大核心
2021年第4期61-65,共5页
Journal of Shaanxi University of Science & Technology
基金
广西南宁市科技型中小企业技术创新资金项目(20183110)。
关键词
复方小钻方颗粒
慢性盆腔炎
抗氧化
抗炎
Xiaozuan Granules
chronic pelvic inflammation
antioxidant
anti-inflammation
