摘要
目的观察电针联合重复经颅磁刺激对阿尔茨海默病(AD)样模型大鼠空间学习记忆能力、突触后致密带、小胶质细胞及神经炎症因子的影响。方法SD大鼠随机分为正常组、模型组、电针组(EA)、重复经颅磁刺激组(rTMS)、电针+重复经颅磁刺激组(EA+rTMS)。采用腹腔注射D⁃半乳糖制备AD样模型大鼠。EA组予电针刺激,20 min/次。rTMS组予每串10个脉冲,共10串的刺激。EA+rTMS组在电针结束后进行经颅磁刺激,方法及参数分别同EA组和rTMS组。干预2周后,进行Morris水迷宫实验、透射电镜采集、免疫组化实验、Western blot实验及数据统计分析。结果与模型组比,各干预组逃避潜伏期缩短(P<0.01);目标象限探索时间延长(P<0.01);各干预组突触后致密物质(PSD)厚度增大(P<0.01);各干预组活化的小胶质细胞减少(P<0.01),TNF⁃α、IL⁃6、IL⁃1β蛋白表达减少(P<0.01)。与EA+rTMS组比,EA组和rTMS组逃避潜伏期延长(P<0.01);目标象限探索时间减少(P<0.01);EA组和rTMS组PSD厚度减小(P<0.01),EA组和rTMS组活化的小胶质细胞增多(P<0.05),TNF⁃α、IL⁃6、IL⁃1β蛋白表达增加(P<0.01)。结论EA联合rTMS能更有效地提高AD样模型大鼠学习记忆能力,可能与抑制神经炎症反应、减少神经元突触损伤有关。
Objective To observe the effects of electroacupuncture(EA)combined with repetitive tran⁃scranial magnetic stimulation(rTMS)on spatial learning and memory ability,postsynaptic dense zone,microglia and neuroinflammatory factors in rats with Alzheimer′s disease(AD).Methods SD rats were randomly divided into normal group,model group,electroacupuncture group,repetitive transcranial magnetic group and electroacu⁃puncture+transcranial magnetic group.AD model rats were prepared by intraperitoneal injection of D⁃galactose.The electroacupuncture(EA)group was given electroacupuncture stimulation for 20 min/times.The repetitive tran⁃scranial magnetic(rTMS)group was given 10 pulses per string,10 strings of stimuli per day.The EA+rTMS group was given magnetic stimulation after electroacupuncture treatment,with the same methods and parameters as in the electroacupuncture and the repetitive transcranial magnetic groups.After 2 weeks of intervention,Morris water maze test,transmission electron microscope collection and analysis,immunohistochemical experiment,Western⁃blot test and data statistical analysis were carried out.Results Compared with the model group,the escape latency in each intervention group was shorter(P<0.01),the time in the target quadrant was significantly prolonged(P<0.01),the thickness of postsynaptic dense zone in each intervention group was significantly larger(P<0.01),the activated microglia and the expression of TNF⁃α,IL⁃6 and IL⁃1βprotein in each intervention group were signif⁃icantly decreased(P<0.01).Compared with the EA+rTMS group,the escape latency of the EA and rTMS groups was significantly longer and the time of target quadrant exploration was significantly shorter(P<0.01),the thick⁃ness of postsynaptic dense zone in the EA and rTMS groups was significantly decreased(P<0.01),the activated microglia in the electroacupuncture group and repeated transcranial magnetic group was significantly increased(P<0.05),and the protein expression of TNF⁃α,IL⁃6 and IL⁃1βwere significa
作者
陈虹茹
何川
黄重生
王颖
孔立红
CHEN Hongru;HE Chuan;HUANG Zhongsheng;WANG Ying;KONG Lihon(College of Acupuncture Moxibustion and Orthopaedics Trau-matology,Hubei University of Traditional Chinese Medicine,Wuhan 430061,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2021年第12期1534-1538,共5页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:81373741)
针灸治未病湖北省协同创新中心/国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程)培育项目(编号:HBPCIC⁃2019⁃06号)。
关键词
重复经颅磁刺激
阿尔兹海默病
学习记忆能力
突触后致密带
小胶质细胞
炎症因子
repetitive transcranial magnetic stimulation
Alzheimer′s disease
learning and memory ability
postsynaptic dense zone
microglia
inflammatory factors
