摘要
【目的】为明确柳叶蜡梅(Chimonanthus Salicifolius)的叶部病害的病原种类,进行病叶病原分离鉴定并研究病原菌株生物学特性。【方法】对福建省寿宁县福瑞泰生物技术有限责任公司种植基地的柳叶腊梅叶斑病病叶采用离体组织分离法进行分离,对分离的病原菌采用形态学结合分子方法(rDNA-ITS和TUB序列)进行鉴定,并对该致病菌菌丝体在不同温度、pH值、光照、碳源、氮源及致死温度等条件下的生物学特性进行研究。【结果】病原菌分离株经形态特征观测及rDNA-ITS和TUB序列在NCBI数据库的分析比对,将寿宁县柳叶腊梅叶斑病病原菌鉴定为子囊菌门,格孢腔目,附球真菌属,高粱附球菌(Epicoccum Sorghinum)。生物学特性结果表明:在5~30℃均可生长,最适生长温度25℃;菌丝体生长最适pH值范围为5~9,在供试的11种碳源上均可生长,最佳碳源为蔗糖;在8种氮源培养基中最适菌丝生长的氮源为蛋白胨;光暗交替不影响菌丝生长,菌丝体致死温度为52℃。【结论】分离鉴定了福建省寿宁县柳叶腊梅叶斑病病害的病原菌为高粱附球菌(E.sorghinum),生物学测定结果显示其对环境适应性强。
【Objective】Pathogen that caused the leaf spot disease on Chimonanthus salicifolius was isolated and classified,and its biological characteristics determined.【Method】Specimens of C.salicifolius plants infected by leaf spot disease at Shouning county in Fujian were collected for in vitro tissue culture in the laboratory.Morphological and molecular examinations were performed on the isolates.Biological properties and culture conditions including temperature,pH,light,carbon source,nitrogen source,and lethal temperature were scrutinized for the pathogenic identification and characterization.【Result】Based on the morphology as well as the rDNA ITS and TUB sequences,the pathogen responsible for the disease was identified to be Ascomycetes Pleosporales Epicoccum sorghinum.It could grow at 5-30℃with an optimum temperature at25℃and pH ranging from 5 to 9 in media of 11 various carbon sources including sucrose and 8 different nitrogen source including peptone under no specific light/dark conditions.The lethal temperature of the fungal mycelia was 52℃.【Conclusion】E.sorghinum was identified to be the major pathogen that caused the leaf spot disease on C.salicifolius in Shouning county.It had strong ability to grow on,resist to,and survive under adverse conditions.
作者
彭成彬
王泽榕
阮俊峰
魏日凤
薛岚
陈美霞
刘伟
PENG Chengbin;WANG Zerong;RUAN Junfeng;WEI Rifeng;XUE Lan;CHEN Meixia;LIU Wei(College of Life Sciences,Ningde Normal University,Ningde,Fujian 352100,China;College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;College of Chemistry and Materials,Ningde Normal University,Ningde,Fujian 352100,China;Fujian Science and Technology Industry-University-Research Cooperation Demonstration Base,Ningde,Fujian 352100,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2021年第4期457-463,共7页
Fujian Journal of Agricultural Sciences
基金
福建省教育厅新世纪优秀人才支持计划项目(闽教科2018-47)
宁德师范学院项目(2018ZX405、2019T02、2020Z01)
福建省自然基金项目(2016N0026)
福建省科技产学项目(2016N5010)。
关键词
柳叶蜡梅
病原菌分离鉴定
多基因序列分析
生物学特性
Chimonanthus salicifolius
pathogenic identification
multiple gene sequence analysis
biological characteristics
