摘要
为明确贵州省纳雍县红托竹荪绿霉病的病原菌种类,并探究杀菌剂对病原菌的室内抑菌效果及红托竹荪菌丝的安全性,以期为红托竹荪绿霉病的田间防治提供理论依据。本研究对贵州省纳雍县乐治镇黄家寨黔产红托竹荪种植基地具有典型症状的红托竹荪绿霉病子实体进行病原菌的分离和纯化,利用科赫氏法则对所得病原菌进行致病性测定,并结合形态学特征、18S rDNA和TEF1基因序列分析及系统发育树进化分析鉴定病原菌株。同时选取了8种不同类型的杀菌剂对病原菌进行室内毒力测定以及杀菌剂对红托竹荪菌丝安全性评价。结果表明:引起红托竹荪绿霉病的病原菌为哈茨木霉(Trichoderma harzianum);室内毒力测定结果显示,对哈茨木霉菌丝生长抑制作用最强的是450 g/L咪鲜胺水乳剂和10%苯醚甲环唑水分散粒剂,其EC50值分别为0.005 3 mg/L和0.000 7 mg/L;8种药剂对红托竹荪的室内安全性测定结果表明,80%代森锌可湿性粉剂、75%百菌清可湿性粉剂对红托竹荪菌丝的抑制率最低,EC50值分别为12.285 4 mg/L和10.893 9 mg/L;其次是25%腐霉·福美双可湿性粉剂,其EC50值为1.845 2 mg/L。综上所述,25%腐霉·福美双可湿性粉剂不仅对红托竹荪绿霉病病原菌有较高的抑制效果,并且对红托竹荪菌丝的安全性也较高,可作为进一步田间试验的首选药剂。
To identify the pathogenic species casuing green mold disease in Dictyophora rubrovalvata in Nayong County,Guizhou Province,and to assess the efficacy of fungicides in inhibiting the pathogen indoors while ensuring the safety of D.rubrovalvata mycelium,this study aims to establish a theoretical foundation for controling D.rubrovalvata green mold disease in the field.In this study,we isolated and purified the pathogenic fungus from the green mold in D.rubrovalvata exhibiting typical symptoms at the D.rubrovalvata planting base in Huangjia Village,Lezhi Town,Nayong County,Guizhou Province.The pathogenicity of the isolated pathogens was subsequently determined by Koch’s method.The pathogenic strains were identified by morphological characterization,analysis of 18S rDNA and TEF 1 gene sequences,and phylogenetic tree evolution.Eight commonly used fungicides were chosen to assess the virulence of the pathogen and evaluate safety of these fungicides on the mycelium of D.rubrovalvata.The study identified Trichoderma harzianum as the pathogenic fungus causing green mold disease in D.rubrovalvata.In indoor virulence assays,the most potent inhibitory effect on D.rubrovalvata growth was observed with 450 g/L prochloraz EW and 10%difenoconazole WG,exhibiting EC 50 values of 0.0053 mg/L and 0.0007 mg/L,respectively.Evaluation of indoor safety for D.rubrovalvata,revealed that 80%zineb WP and 75%chlorothalonil WP exhibited the lowest inhibition rate on D.rubrovalvata mycelium,with EC 50 values of 12.2854 mg/L and 10.8939 mg/L,respectively,followed by 25%procymidone In summary,25%procymidone Thiram WP exhibited potent inhinition against the D.rubrovalvata green mold disease,while also demonstrating a favorable safety profile for D.rubrovalvata mycelium.This makes it a promising candidate for further field trials.
作者
滕虎亮
秦顺
王俊容
戴仁怀
宋理洪
张钰龙
刘增亮
陈孝玉龙
Teng Huliang;Qin Shun;Wang Junrong;Dai Renhuai;Song Lihong;Zhang Yulong;Liu Zengliang;Chen Xiaoyulong(College of Agriculture,Guizhou University,Guiyang 550025,Guizhou,China;College of Resource and Environmental Engineering,Guizhou University,Guiyang 550025,Guizhou,China;Microbiology Research Institute,Guangxi Academy of Agriculture Sciences,Nanning 530007,Guangxi,China;College of Life Sciences,Guizhou University,Guiyang 550025,Guizhou,China)
出处
《山地农业生物学报》
2024年第4期31-38,共8页
Journal of Mountain Agriculture and Biology
基金
贵州省科技计划项目(黔科合支撑[2020]1Y124)
广西科技基地和人才专项(桂科AA21196003)
贵州大学实验室开放项目(SYSKF2023-093)。
关键词
红托竹荪
哈茨木霉
绿霉病
药剂筛选
Dictyophora rubrovalvata
Trichoderma harzianum
green mold disease
fungicide screening
