摘要
鼓粒期是大豆碳氮代谢最复杂的阶段,干旱胁迫必然限制鼓粒期大豆碳氮同化、分配和转移,影响大豆产量的形成。在我们前期的研究中,明确了外源褪黑素对干旱胁迫下鼓粒期大豆抗旱和碳氮代谢的生理调控效应。本研究通过转录组和代谢组分析来确定褪黑素对大豆干旱条件反应的一些重要的碳氮代谢基因和途径。转录组分析表明,与干旱胁迫处理相比,正常供水和干旱胁迫下喷施外源褪黑素处理的大豆叶片共同上调和下调的基因分别有37个和493个。上调的基因中存在着直接和间接参与碳氮代谢的功能基因,包括正向调控的参与半胱氨酸合成、光合作用、碳水化合物代谢和葡萄糖代谢等途径关键基因。代谢组分析发现,与干旱胁迫处理相比,正常供水和干旱胁迫下喷施外源褪黑素处理的大豆叶片共同上调和下调的代谢物分别有17个和43个,上调的代谢物中绝大部分(14/17)属于氨基酸、脂质、有机酸和碳水化合物,进一步揭示了外源褪黑素能够提高大豆碳氮代谢与抗旱的能力。结合转录组和代谢组分析发现,褪黑素通过调节氨基酸代谢和淀粉蔗糖代谢途径,促进干旱胁迫下b-葡萄糖苷酶基因表达,提高了L-天冬酰胺和6-磷酸葡萄糖代谢物的含量,最终提高了大豆的抗旱性。
The grain-filling stage is the most complex stage of carbon and nitrogen metabolism.Drought stress inevitably inhibits the assimilation,distribution,and transition of carbon and nitrogen at grain-filling stage in soybean,resulting in less soybean yield.The objective of this study was to investigate the effects of exogenous melatonin on the carbon and nitrogen metabolism genes and pathways under drought stress in soybean.Transcriptome analysis showed that,compared with drought stress treatment,37 and 493 genes were jointly up-regulated and down-regulated in soybean leaves treated with normal water supply and treated with exogenous melatonin under drought stress,respectively.The up-regulated genes included functional genes directly and indirectly involved in carbon and nitrogen metabolism,such as the key genes involved in the cysteine synthesis pathway,photosynthesis,carbohydrate metabolism,and glucose metabolism.Metabolomic analysis revealed that,compared with drought stress treatment,17 and 43 metabolites were jointly up-regulated and down-regulated in soybean leaves treated with normal water supply and treated with exogenous melatonin under drought stress,respectively.Most(14/17)of up-regulated metabolites were amino acids,lipids,organic acids,and carbohydrates,which further indicated that exogenous melatonin could improve soybean carbon and nitrogen metabolism and drought resistance in soybean.Combined with transcriptome and metabolomic profile,melatonin promoted the relative expression level of β-D-Glucosidase gene due to regulate the pathway of amino acid metabolism and starch and sucrose metabolism,improved the contents of L-Asparagine and D-glucose-6P metabolites,and ultimately improves the ability of drought resistance in soybean.
作者
曹亮
杜昕
于高波
金喜军
张明聪
任春元
王孟雪
张玉先
CAO Liang;DU Xin;YU Gao-Bo;JIN Xi-Jun;ZHANG Ming-Cong;REN Chun-Yuan;WANG Meng-Xue;ZHANG Yu-Xian(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第9期1779-1790,共12页
Acta Agronomica Sinica
基金
国家重点研发计划项目“大田经济作物优质丰产的生理基础与调控”(2018YFD1000905)
黑龙江省应用技术研究与开发计划项目(GA19B101-02)
黑龙江省农垦总局重点科研计划项目(HKKY190206-01)资助
关键词
褪黑素
大豆
鼓粒期干旱
碳氮代谢
代谢组和转录组
melatonin
soybean
drought at grain-filling stage
carbon and nitrogen metabolism
metabolome and transcriptome
