摘要
为了建立快速检测非洲猪瘟病毒(African swine fever virus,ASFV)的血清学方法,本研究原核表达了ASFV的D1133L重组蛋白,并以其为包被抗原,通过反应条件优化,建立了一种快速检测ASFV的间接ELISA方法,并采用该方法检测了ASFV感染猪后D1133L的抗体应答情况。结果显示:成功表达纯化了D1133L蛋白,以此为抗原建立了ELISA抗体检测方法,该方法特异性高,灵敏性强,其检测结果与市售试剂盒检测结果的符合率达100%。家猪在感染ASFV后,针对D1133L的抗体在第5天可被检测到,在第7天抗体水平达到顶峰。
To establish a serological method for the rapid detection of African swine fever virus(ASFV),prokaryotic expression of ASFV D1133L recombinant protein was used as the encapsulated antigen.A rapid indirect ELISA method for detection of ASFV was established by optimizing the reaction conditions,and the D1133L antibody response of pigs infected with ASFV was detected by this method.The results showed that D1133L protein was successfully expressed and purified,and an ELISA antibody detection method with high specificity and sensitivity was established.The coincidence rate with the marketing kit was 100%.After infection with ASFV,antibodies to D1133L were detected on day 5,and antibody levels peaked on day 7.
作者
侯景
申超超
张大俊
杨博
史喜绢
张婷
杨泽晓
张克山
郑海学
李丹
党文
刘湘涛
HOU Jing;SHEN Chao-chao;ZHANG Da-jun;YANG Bo;SHI Xi-juan;ZHANG Ting;YANG Ze-xiao;ZHANG Ke-shan;ZHENG Hai-xue;LI Dan;DANG Wen;LIU Xiang-tao(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;State Key Laboratory of Veterinary Etiological Biology,National Foot and Mouth Diseases Reference Laboratory,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)
出处
《江西农业学报》
CAS
2021年第6期1-6,共6页
Acta Agriculturae Jiangxi
基金
国家自然科学基金联合项目(31941002)
国家重点研发计划(2018YFC0840400)
广东省非洲猪瘟科技应急防控专题(20190211)。
