摘要
[目的]验证α-硫酸钙(α-calcium sulphate hemihydrate,α-CSH)/纳米纤维素(nanocellulose,NC)复合植骨材料修复骨缺损的性能。[方法]采用单向轴压模具制作直径为6mm,高度8mm的圆柱状植骨材料,消毒后备用。12只新西兰兔随机分组,于股骨髁制备骨缺损,空白组不植入任何材料,α-CSH组及α-CSH/NC组分别向骨缺损处植入相应材料。于术后4、12周处死动物并取材,行Micro-CT检测和组织形态观察。[结果]Micro-CT扫描显示,术后4周α-CSH/NC组植入物残留体积为53.02%,而α-CSH组的残存体积为5.54%,差异具有显著统计学意义(P<0.05)。12周时经Micro-CT骨形态计量,α-CSH/NC组新生骨在骨表面积、骨体积、相对骨体积分数、骨小梁分离度等方面均显著优于α-CSH组和空白组,差异具有统计学意义(P<0.001)。组织学观察所见与Micro-CT扫描形态观察结果一致。[结论]与单纯α-CSH材料相比,α-CSH/NC复合材料植入骨缺损后其被吸收显著延缓,成骨效应显著改善。α-CSH/NC复合材料可能是一种优良的骨移植替代材料。
[Objective] To verify the performance of α-calcium sulphate hemihydrate(α-CSH)/nanocellulose(NC) compound for repairing bone defects in rabbits. [Methods] The α-CSH and α-CSH/NC compound were made to a cylindrical bone graft material with the diameter in 6 mm and height in 8 mm by using a unidirectional axial compression, and then was sterilized for further use. Twelve New Zealand rabbits were randomly divided into groups, and bone defects corresponding to the implant in size were created in the femoral condyles.No material was implanted into the bone defect in the blank group, while corresponded materials were implanted into the bone defects in the animals of α-CSH group and the α-CSH/NC group. At 4 weeks and 12 weeks after the operation, the animals were sacrificed and the femoral sample were harvested for Micro-CT assay and histological observation. [Results] Micro-CT scan showed that the residual volume of the implant was 53.02% in the α-CSH/NC group, whereas 5.54% in the α-CSH group at 4 weeks after operation, which was statistically significant(P<0.05). Regarding to micro-CT bone morphometry at 12 weeks postoperatively, the α-CSH/NC group was significantly superior to the α-CSH group and the blank group in terms of new bone volume fraction, trabecular bone thickness, and trabecular separation,which were statistically significant(P<0.001). In addition, the histological observations were consistent with the micro-CT scan morphological findings. [Conclusion] Compared with the pure α-CSH material, the absorption of the α-CSH/NC composite material is significantly delayed after implantation into the bone defect, while its osteogenic capacity is significantly improved. The α-CSH/NC composite implant might be an excellent bone graft substitute material.
作者
刘金龙
孙晓彤
张玉娟
杜庆
孙刚和
刘相成
LIU Jin-long;SUN Xiao-tong;ZHANG Yu-juan;DU Qing;SUN Gang-he;LIU Xiang-cheng(Department of Orthopedics,Hospital of 80^(th) Group Army,PLA,Weifang 261042,China;School of Anesthesiology,Weifang Medical College,Weifang 261053,China)
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2021年第10期920-924,共5页
Orthopedic Journal of China
基金
潍坊市卫生健康委员会科研资助项目(编号:wfwsjk2019007)。
关键词
α-硫酸钙
纳米纤维素
植骨材料
骨缺损修复
动物实验
α-calcium sulfate hemihydrate
nanocellulose
bone graft material
bone defect repair
animal experiment
