摘要
背景:组织工程骨为负重部位大段骨缺损修复和重建提供了巨大潜力,目前关于组织工程骨应用研究策略主要集中于支架复合细胞或生物活性分子,而骨组织工程支架的结构化和功能化是其中最重要的研究内容之一。目的:利用3D打印制备兔胫骨解剖型支架,建立一种简便易行解剖形状可塑的组织工程骨骨缺损模型。方法:采用Mimic软件获得缺损骨的三维电子模型,利用熔融沉积制法备羟基磷灰石/聚己内酯解剖型支架。将15只6月龄新西兰大白兔随机分为3组,空白组3只,对照组和实验组均6只,分别制备长1.2 cm的胫骨完全骨缺损,其中空白组缺损处旷置,对照组缺损处将截骨原位放回,实验组缺损处放置解剖型组织工程骨,并用实验设计的小钢板螺钉固定。结果与结论:(1)兔胫骨测量结果:兔胫骨长(93.77±0.59)mm;胫腓交界横径(8.36±0.13)mm,矢状径(5.97±0.12)mm,骨皮质平均厚度(1.20±0.10)mm,髓腔平均直径(4.30±0.06)mm;胫骨模型侧面上下关节面中点连线与截骨模型侧面上下截面中点连线的夹角α=(5.97±0.13)°;(2)缺损区X射线显示:术后4,12周,实验组和对照组骨缺损处移植体未见明显移位和成角畸形,自体骨修复较好;(3)组织学检查:术后4周,实验组骨断端及支架内有少量新生骨填充,术后12周,重建支架内新骨生成明显增多并且部分矿化;(4)一般观察:术后4,12周,实验组和对照组组骨缺损处骨移植体无明显移位、成角畸形,实验组和对照组夹角(α)测量差异无显著性意义。(5)结果证实:实验采取的3D打印解剖形状可塑的组织工程骨构建的兔胫骨大段完全骨缺损模型稳定性较好,可模拟骨组织的结构和特异性指导新骨的再生和重建。
BACKGROUND：Tissue-engineered bone has been considered to be a promising candidate for the repair and reconstruction of load-bearing large segmental bone defects.Currently,the studies on the application of tissue-engineered bone mainly focus on cell-scaffold or cytokine-scaffold constructs,which have shed light upon the repair of large segmental bone defects.OBJECTIVE：To establish simple and convenient tissue engineering of anatomically shaped tibial bone defect models using three-dimensional rapid prototyping technology to manufacture rabbit tibia biomimetic artificial bone scaffolds.METHODS：Three-dimensional electronic models were constructed using Mimic software.Hydroxyapatite/polycaprolactone scaffolds were manufactured by fused deposition modeling equipment.Fifty rabbits aged 6 months were randomly divided into three groups：blank control（n=3）,control（n=6） and experimental groups（n=6）,respectively.Tibial defects ranged 1.2 cm were made in all groups.No treatment was given in blank control group.The bone defects in control and experimental groups were repaired with autogenous osteotomized bone and anatomical tissue-engineered bone,respectively,and fixed with plates and screws.RESULTS AND CONCLUSION：（1） Rabbit tibial bone measurements：tibial length was（93.77±0.59） mm,tibiofibular transverse diameter（8.36±0.13） mm,sagittal diameter（5.97±0.12） mm,average thickness of bone cortex（1.20±0.10） mm,average diameter of the medullary cavity（4.30±0.06） mm.Angle between the connection line of the midpoints of superior and inferior articular surfaces at the side of tibial bone models and the connection line of the midpoints of superior and inferior intersecting surfaces at the side of osteotomized bone models was α=（5.97±0.13）°.（2） X-ray in bone defects：at postoperative 4 and 12 weeks,no obvious displacement and angulated deformity were found in bone grafts,suggesting the good bone defect repair.（3） Histological examination：at postoperative 4 weeks,bone
出处
《中国组织工程研究》
CAS
北大核心
2016年第24期3508-3515,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金面上项目(81271954)
上海市科委科技支撑项目(15411951000)~~
