摘要
目的观察丹参饮预处理对心肌缺血/再灌注损伤(MI/RI)大鼠心肌梗死面积、心功能和炎症因子的影响。方法超高效液相色谱-高分辨质谱联用分析法分析丹参饮的化学成分;将Wistar大鼠随机分为假手术组、模型组、丹参饮低剂量预处理I/R组、丹参饮中剂量预处理I/R组、丹参饮高剂量预处理I/R组;给药1周后,采用大鼠冠状动脉前降支结扎30 min/复流24 h的方法建立心肌缺血/再灌注模型;2,3,5-氯化三苯基四氮唑染色观察心肌梗死面积,ImageJ软件计算心肌梗死面积;使用心脏彩色多普勒仪观察各组大鼠左室射血分数(LVEF)和左室缩短率(LVFS);采用ELISA法测定各组大鼠血清白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的表达。结果 (1)丹参饮的主要化学成分为丹酚酸B、丹参酮ⅡA、槲皮素、槲皮苷。(2)与模型组比较,丹参饮各剂量组大鼠心肌梗死面积均有下降(P<0.05)。(3)与假手术组比较,模型组大鼠的LVEF和LVFS均明显降低(P<0.01);与模型组比较,丹参饮各剂量预处理I/R组大鼠的LVEF和LVFS均有不同程度改善(P<0.05,P<0.01)。(4)与假手术组比较,模型组大鼠血清中IL-6和TNF-α含量均有增加(P<0.01);与模型组比较,丹参饮各剂量预处理I/R组大鼠血清IL-6水平均明显下降(P<0.01),丹参饮低、中剂量预处理I/R组TNF-α水平亦均有下降(P<0.05)。结论丹参饮预处理具有减少MI/RI大鼠心肌梗死面积、改善心功能的作用,其机制可能与减少缺血再灌注时血清炎症因子的表达相关。
Objective To observe the effect of Danshen Decoction(DSY) pretreatment on infarct size, cardiac function and inflammatory cytokines in serum in rats with myocardial ischemia/reperfusion injury(MI/RI). Methods Chemical components of DSY were analyzed by ultra-high performance liquid chromatography/ultra-resolution mass spectrometry. Thirty Wistar rats were randomly divided into five groups: sham operation group, ischemia/reperfusion(I/R) model group, DSY(low-dose, medium-dose and high-dose) pretreatment I/R groups, with 6 rats in each group. After 1 week of continuous gavage, the rat model of myocardial ischemia/reperfusion(I/R) was established by ligation of the anterior descending coronary artery for 30 min/reflow for 24 h. TTC staining was used to observe the infarct size, which was measured by ImageJ software. Left ventricular ejection fraction(LVEF) and left ventricular fraction shortening(LVFS) were observed by Cardiac Color Ultrasound Doppler. The levels of interleukin-6(IL-6) and tumor necrosis factor-α( TNF-α) in serum of each rat group were determined by ELISA. Results(1)The main chemical components of DSY were salvianolic acid B,tanshinone IIA,quercetin and quercitrin.(2) Compared with the model group, the size of myocardial infarction was decreased in DSY( low-dose,mediumdose and high dose) preconditioning groups( P < 0. 05).(3)Compared with sham operation group,LVEF and LVFS in I/R model group were significantly decreased( P < 0. 05,P < 0. 01). Compared with I/R model group,the LVEF and LVFS of rats in DSY( low-dose,medium-dose and high dose) preconditioning groups were improved( P<0.05).(4)Compared with the sham operation group,the levels of IL-6 and TNF-α in serum in I/R model group were increased( P <0.01). Compared with I/R model group,the levels of IL-6 in serum in DSY( low-dose,mediumdose and high dose) pretreatment groups were decreased( P<0.01). The levels of TNF-α in serum in DSY( low-dose and medium-dose) pretreatment groups were also decreased( P<0.05). There was no significant differen
作者
呼明哲
李湘玲
杨涛
王敏燕
王肖龙
高俊杰
HU Mingzhe;LI Xiangling;YANGTao;WANG Minyan;WANG Xiaolong;GAO Junjie(department of cardiology,shuguang hospital affiliated to shanghai university of traditional chinese medicine,shanghai 201203,china;institute of cardiovascular diseases,shuguang hospital affiliated to shanghai university of traditional chinese medicine,shanghai 201203,china;department of pathology,shuguang hospital affiliated to shanghai universily of traditional chinese medicine,shanghai 201203,chinn)
出处
《上海中医药杂志》
2021年第6期61-66,共6页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81703743)。
关键词
丹参饮
心肌缺血/再灌注损伤
心功能
炎症因子
心肌保护
Danshen Decoction
myocardial ischemia/reperfusion injury
cardiac function
inflammatory cytokines
cardioprotection
