摘要
目的探讨微小RNA-195(miR-195)在弥漫大B细胞淋巴瘤(DLBCL)细胞中的表达变化,并探讨miR-195过表达对瘤细胞增殖、凋亡、侵袭及迁移等肿瘤生物学特性的影响及可能的作用机制。方法采用实时荧光定量PCR检测人DLBCL细胞系U2932、HBL1、OCI-LY-10及人正常B淋巴细胞系IM-9中的miR-195。将OCI-LY-10细胞随机分为miR-195 mimics组和NC-mimics组,分别转染miR-195 mimics和NC-mimics,采用实时荧光定量PCR检测miR-195,采用CCK-8实验检测细胞增殖,采用流式细胞术检测细胞凋亡,采用Transwell实验检测细胞侵袭和迁移能力,采用Westernblotting法检测HMGA2蛋白。结果与IM-9比较,U2932、HBL1、OCI-LY-10细胞中miR-195相对表达量降低(P均<0.05),且在OCI-LY-1中最明显。与NC-mimics组比较,miR-195 mimics组miR-195相对表达量高,细胞增殖活性低,细胞凋亡率高,侵袭及迁移穿膜细胞数减少,HMGA2蛋白表达低(P均<0.05)。结论miR-195在DLBCL细胞中呈低表达;上调DLBCL细胞中miR-195的表达可以抑制瘤细胞的增殖、侵袭和迁移,增加细胞的凋亡,其机制可能与miR-195对HMGA2的靶向调控有关。
Objective To investigate the expression of microRNA-195(miR-195)in diffuse large B-cell lymphoma(DLBCL)cells,and to explore the effect of miR-195 overexpression on the biological characteristics such as proliferation,apoptosis,invasion and migration of tumor cells and its possible mechanism.Methods The qRT-PCR was used to detect the expression of miR-195 in human DLBCL cell line U2932,HBL1,OCI-LY-10 and human normal B lymphocyte line IM-9.OCI-LY-10 cells were randomly divided into miR-195 mimics group and mimics-NC group,and miR-195 mimics and mimics-NC were transfected into OCI-LY-10 cells,respectively.The qRT-PCR was used to detect the expression of miR-195,CCK-8 assay was used to detect cell proliferation,flow cytometry was used to detect apoptosis,Transwell assay was used to detect cell invasion and cell migration,and Western blotting was used to detect HMGA2 protein.Results Compared with IM-9,the relative expression of miR-195 in U2932 cells,HBL1 cells and OCI-LY-10 cells decreased(all P<0.05),andwasmostobviousinOCI-LY-10cells.ComparedwiththeNC-mimicsgroup,themiR-195mimicsgrouphad higher relative expression of miR-195,lower cell proliferation activity,higher apoptosis rate,less invasion and migration of transmembrane cells,and lower expression of HMGA2 protein(all P<0.05).Conclusions The expression of miR-195 is low in DLBCL cells.Up-regulation of miR-195 expression in DLBCL cells can inhibit cell proliferation,cell invasion and cell migration,and increase apoptosis.The mechanism may be related to the targeted regulation of HMGA2.
作者
盛秀云
张磊
王艳军
SHENG Xiuyun;ZHANG Lei;WANG Yanjun(Liaocheng Second People's Hospital,Liaocheng 252600,China)
出处
《山东医药》
CAS
2021年第16期52-55,共4页
Shandong Medical Journal
基金
山东省自然科学基金资助项目(ZR2017BH056)。
