摘要
番茄灰霉病是由灰葡萄孢菌(Botrytis cinerea)导致的世界性真菌病害,通过诱导抗病防治具有环保可持续等优点。针对具有诱抗潜力的绿针假单胞菌(Pseudomonas choloeaphtis)PA6及β-氨基丁酸,明确两种预处理对番茄灰霉病的防治效果及对根际细菌群落结构的影响。主要采用盆栽试验及实时荧光定量技术测定不同预处理对灰霉病诱抗效果及番茄抗性基因表达情况,通过16S rDNA高通量测序分析根际细菌群落结构变化。结果表明:绿针假单胞菌及β-氨基丁酸处理都可降低番茄植株发病率,分别使病情指数下降60.4%和36.6%,并促进抗性基因NPR1和PR1表达,激发SA信号通路。高通量测序结果表明:预处理增加根际细菌中变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)及拟杆菌门(Bacteroidetes)的丰度,调整番茄根际细菌的优势菌属丰度及结构,根际益生菌群丰度显著上升。
Tomato grey mould is a worldwide fungal disease caused by Botrytis cinerea.Induced resistance has the advantages of environmental protection and sustainability.In this paper,Pseudomonas choloeaphtis andβ-amino butyric acid with potential for resistance were used to determine the anti-disease effect of tomato gray mold and the regulation of rhizosphere bacterial community structure by two pretreatments.The pot test and real-time fluorescence quantitative technology were used to obtain the resistance to gray mold and tomato resistance gene expression under different pretreatments.The 16S rDNA high-throughput sequencing was used to investigate the rhizosphere bacterial community structural change.The results showed that both Pseudomonas choloeaphtis andβ-aminobutyric acid treatment could reduce the incidence of tomato plants with reducing the disease index by 60.4%and 36.6%respectively,promoted the expression of resistance genes NPR1 and PR1,and stimulated the SA signaling pathway.High-throughput sequencing results showed that pretreatment increased the abundance of Proteobacteria,Pachyphyta and Bacteroides in rhizosphere bacteria,adjusted the abundance and structure of the dominant genus of tomato rhizobacteria.The abundance of probiotic flora in rhizosphere increased significantly.
作者
苏莹莹
金朝霞
崔宇兴
SU Yingying;JIN Zhaoxia;CUI Yuxing(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2021年第3期73-77,共5页
Journal of Biology
基金
国家自然科学基金项目(31670604)
辽宁省自然科学基金项目(20180550237)。
关键词
灰霉病
高通量测序
番茄
根际微生物
抗性基因
gray mold
high-throughput sequencing
tomato
rhizosphere microorganisms
resistance genes
