摘要
【目的】探究4种新疆本土草本植物生物修复含油污泥的潜力,及其与微生物联合修复对低浓度含油污泥处理效果。【方法】以狗牙根(Cynodondactylon)、高羊茅(Festuca elata)、黑麦草(Lolium Perenne)和苏丹草(Sorghum sudanense)与解脂假丝酵母(Candida lipolytica,菌A)、2株枯草芽孢杆菌[菌B(Bacillus subtilis PL-2)和菌C(Bacillus subtilis XJ-16)]、混菌D(A+B+C)为研究对象,采用盆栽试验,分别设置微生物组、植物组、植物-微生物组及对照4组处理,分析不同处理对石油烃残留量、微生物数量、草种生物量和叶绿素含量的影响。【结果】120 d盆栽试验结表明,单独种植狗牙根、单独种植苏丹草、菌B、菌C处理对石油烃的降解效果较为相近,降解率分别为31.39%、34.19%、33.71%与33.39%,显著高于对照组(P<0.05);草本植物与微生物联合修复中,狗牙根中添加菌A、菌B和菌C效果显著,石油烃的降解率可达43.02%、40.20%和42.54%,且在联合修复后土壤中可培养细菌与真菌数量显著增加,分别为1.50×10^(5)~2.59×10^(5)cfu·g^(-1)和4.32×10^(4)~5.53×10^(4)cfu·g^(-1);此外,在添加降解菌后,狗牙根干重和叶绿素总含量均显著提高(P<0.05)。【结论】综合石油烃残留量、微生物数量、生物量以及叶绿素含量指标得出,新疆本地植物狗牙根与菌A(解脂菌假丝酵母)、菌B和菌C(均为枯草芽孢杆菌)的联合,可为当地低浓度含油污泥处理提供可行的技术方案。
【Objective】Potential of applying native Xinjiang plants in combination with microbes as a bioagent to treat low concentration oil sludge pollution was explored.【Method】Three treatments of microbes,plants,and plant-microbe combination that used plant including Cynodon dactylon,Festuca elata,Lolium Perenne,or Sorghum sudanense and the microbes including Candida lipolytica(Microbe A),Bacillus subtilis PL-2(Bacterium B),and/or B.subtilis XJ-16(Bacterium C)were conducted along with control in a pot experiment for 120 d.Effects of the treatments on petroleum hydrocarbon residues,and microbial population,as well as biomass and chlorophyll content of the plants,were determined.【Result】Under the single-factor treatments,C.dactylon,S.sudanense,Bacterium B,or Bacterium C provided relatively similar effect in degrading petroleum hydrocarbons with 31.39%,34.19%,33.71%,and 33.39%reductions,respectively,which were significantly higher than control(P<0.05).The plant-microbe combinations,on the other hand,showed significantly greater pollution remedying effect,such as achieved by incorporating C.dactylon with Bacterium A,B,and C that delivered the hydrocarbon removal rates of 43.02%,40.20%,and 42.54%,respectively.Meanwhile,the culturable bacteria count increased significantly from 1.50×10^(5)cfu·g^(-1)to 2.59×10^(5)cfu·g^(-1)and fungi count from 4.32×10^(4)cfu·g^(-1)to 5.53×10^(4)cfu·g^(-1).In addition,the dry weight and total chlorophyll content of C.dactylon rose significantly in the presence of the microbes(P<0.05).【Conclusion】In view of petroleum hydrocarbon residue,microbial population,plant biomass,and leaf chlorophyll content,it appeared that the combined applications of Xinjiang native plant C.dactylon with yeast C.lipolytica and bacterium B.subtilis PL-2 or XJ-16 could be feasible for cleaning the environmental pollution caused by low concentration oil sludge in the area.
作者
何飞焱
施宠
董丁
季凯
韩斯琴
张颖
HE Feiyan;SHI Chong;DONG Ding;JI Kai;HAN Siqin;ZHANG Ying(College of Resource and Environmental Sciences,Xinjiang Agricultural University,Urumqi,Xinjiang 830052,China;Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang,Liaoning 110016,China;Xin’aoda Petroleum Technology Service Co.,Ltd.,Karamay,Xinjiang 834000,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2022年第10期1344-1353,共10页
Fujian Journal of Agricultural Sciences
基金
中国科学院科技服务网络计划(STS)重点项目(KFJ-STS-ZDTP-064)
国家自然科学基金项目(31760704)
关键词
植物-微生物联合修复
石油污染
降解率
微生物数量
生理特性
Microbe-mediated phytoremediation
petroleum contamination
degradation rate
microbial counts
physiological property
