摘要
[目的]采用高效液相色谱-荧光法测定药桑不同部位1-脱氧野尻霉素(1-DNJ)含量。[方法]用AQC为衍生试剂进行衍生化反应,流动相为乙腈-乙酸铵(85∶15),流速1.0 mL/min,进样量10μL,最大激发波长250 nm,最大发射波长为395 nm下进行荧光检测;用正交试验考察最佳提取工艺条件。[结果]通过正交试验,得到最佳提取工艺为65%乙醇、料液比1∶150、超声功率150 W、提取次数2次、超声时间10 min,在最佳提取工艺下,桑叶、果实、桑枝中1-DNJ的含量分别是1.069%、0.341%、0.078%。[结论]高效液相色谱-荧光检测法操作简便、灵敏度高、准确可靠,可用于药桑不同部位的1-DNJ含量测定。
[Objective]To determine the content of 1-DNJ in different parts of mulberry by high performance liquid chromatography-fluorescence method.[Method]AQC was used as the derivative reagent to conduct the derivative reaction and generate the fluorescence product.The fluorescence detection was performed by HPLC at the maximum excitation wavelength of 250 nm and the maximum emission wavelength of 395 nm.The optimal mobile phase was ammonium acetonitrile acetate(85∶15),flow rate of 1.0 mL/min,and sample injection volume of 10μL.Orthogonal experiment was used to investigate the optimal extraction process conditions.[Result]Through orthogonal experiment,the best extraction process was 65%ethanol,solid-liquid ratio 1∶150,ultrasonic power 150 W,extraction times 2 times,ultrasonic time 10 min.Under the best extraction process,the content of 1-DNJ in mulberry leaves,fruits,and mulberry branches was 1.069.%,0.341%,0.078%,respectively.[Conclusion]The high performance liquid chromatography-fluorescence detection method was simple,sensitive,accurate and reliable,and could be used for the determination of 1-DNJ content in different parts of mulberry.
作者
伊丽则热·艾拜杜拉
陈冰婷
李德龙
谭惠文
马晓丽
ABAYDULA Yilzera;CHEN Bing-ting;LI De-long(College of Pharmacology,Xinjiang Medical University,Urumqi,Xinjiang 830011)
出处
《安徽农业科学》
CAS
2021年第11期178-181,204,共5页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(81760753)
新疆维吾尔自治区自然科学基金项目(2018D01C170)。
关键词
药桑
1-脱氧野尻霉素
提取工艺
优化
含量测定
正交试验
高效液相色谱-荧光检测
Mulberry
1-DNJ
Extraction process
Optimization
Content determination
Orthogonal test
High performance liquid chromatography-fluorescence detection
