摘要
转录因子X盒结合蛋白1(X-box binding protein-1,XBP1)在未折叠蛋白反应中起关键作用。本研究旨在分析XBP1在小鼠早期妊娠过程中的表达和调节,探讨其与胚胎着床的相关性。分别建立小鼠早期妊娠、人工诱导蜕膜化、发情周期和激素调节模型,通过免疫组织化学染色和实时定量RT-PCR技术检测XBP1在这些动物模型中的表达模式。结果显示,XBP1在小鼠早期妊娠子宫中呈现动态的变化,第1~4天主要表达在腔上皮和腺上皮,第5~8天主要表达在蜕膜区;在人工诱导蜕膜化的子宫中XBP1主要存在于蜕膜区;在小鼠发情周期模型中,Xbp1、Xbp1u、Xbp1s mRNA在发情前期的表达量最高;在卵巢切除的子宫中,雌激素处理后腔上皮和腺上皮细胞XBP1的表达上调。以上结果表明,XBP1参与小鼠胚胎着床和蜕膜化,其在早期妊娠子宫腔上皮和腺上皮中的表达受到雌激素调控。
The transcription factor X-box binding protein-1(XBP1)plays a key role in unfolded protein reaction.This study was aimed to investigate the expression pattern and regulation of XBP1 in the mouse uterus during early pregnancy.The methods of immunohistochemistry(IHC)and real time quantitative RT-PCR were used to test XBP1 expression in early pregnancy,artificial decidualization,oestrous cycle and hormone-regulated mouse models.The results showed that XBP1 was spatiotemporally expressed in mouse uterus during early pregnancy.The XBP1 protein was mainly detected in the luminal and glandular epithelia on days 1–4 of pregnancy,and was strongly detected in the decidual area on days 5–8 of pregnancy.Similarly,XBP1 expression was also mainly expressed in decidual cells following artificial decidualization.During the oestrous cycle,Xbp1,Xbp1 u,and Xbp1 s mRNA was predominantly present in proestrus.In the ovariectomized uterus,the expression of XBP1 in luminal and glandular epithelia was up-regulated after estrogen treatment.These results suggest that XBP1 is associated with embryo implantation and decidualization during early pregnancy in mice,and the expression of XBP1 in luminal and glandular epithelia may be regulated by estrogen.
作者
郭思维
于雯
王煦
刘健慧
张新月
何成强
丁乃峥
GUO Si-Wei;YU Wen;WANG Xu;LIU Jian-Hui;ZHANG Xin-Yue;HE Cheng-Qiang;DING Nai-Zheng(Key Laboratory of Animal Resistance Biology of Shandong Province,College of Life Sciences,Shandong Normal University,Jinan 250014,China)
出处
《生理学报》
CAS
CSCD
北大核心
2021年第2期208-216,共9页
Acta Physiologica Sinica
基金
supported by the National Natural Science Foundation of China(No.31771659,31271536)。
关键词
小鼠
X盒结合蛋白1
早期妊娠
子宫
胚胎着床
mouse
X-box binding protein-1
early pregnancy
uterus
embryo implantation
