摘要
目的探究低剂量鸢尾素在小鼠白色脂肪组织(WAT)棕色化及棕色脂肪组织(BAT)的作用以及对饮食诱导的肥胖代谢功能的影响。方法选择30只C57/BLKS/J雄性小鼠,分别进行正常饮食喂养(NCD)和高脂饮食喂养(HFD)。将两种小鼠分别分成实验组和对照组,实验组每日腹腔注射鸢尾素(0.8 ng/g,200μl),对照组每日给予同等体积(200μl)磷酸盐缓冲液,连续干预14 d。每日定时采集小鼠的食物摄入量及体重等指标。干预结束后处死小鼠进行取材,通过组织病理学和免疫组织化学方法检测脂肪组织脂泡大小、活性改变。分析特异性棕色化解偶联蛋白1(UCP1)的表达量来评估不同浓度(0、20和40 nmol/L)鸢尾素在体外对白色及棕色脂肪细胞的干预效果。为评估鸢尾素是否具有改善代谢的功能,对HFD的小鼠进行血清学指标和肝脂肪变性的监测。结果在体外成功培养并鉴定原代白色及棕色脂肪细胞。NCD小鼠中,动物活体显示在接受干预后两组小鼠体重改变值差异有统计学意义[实验组比对照组为(-0.78±0.98)比(0.27±0.55)g,P<0.01]。离体组织病理显示,实验组白色脂肪细胞面积较小[实验组比对照组为(14.78±8.44)比(29.49±12.97)μm2,P<0.05];实验组棕色脂肪细胞内的脂滴较大,且UCP1表达量高于对照组。HFD小鼠中,实验组血糖和胆固醇水平均低于对照组[实验组比对照组分别为(7.18±0.41)比(13.48±2.07)mmol/L,P<0.01;(2.38±0.26)比(3.89±0.93)mmol/L,P<0.05],实验组肝细胞内脂滴含量低于对照组[实验组比对照组为(2.73±1.96)%比(14.04±6.29)%,P<0.001]。结论低剂量鸢尾素能够促进小鼠白色脂肪棕色化,同时能激活BAT,从而产热减轻小鼠体重;并且能够改善饮食引起的小鼠肥胖和相关的代谢紊乱。
Objective To explore the role of low-dose irisin in the browning of white adipose tissue(WAT)and activation of brown adipose tissue(BAT)in mice,and its effect on the metabolic function of diet induced obesity.Methods A total of 22 C57/BLKS/J male mice fed with normal diet and 8 fed with high fat diet were separately divided into experimental and control group.The experimental group was given irisin(0.8 ng/g,200μl),while the control group was given the same volume(200μl)of phosphate buffer saline every day for 14 consecutive days intraperitoneally.Food intake and body weight of mice were collected regularly every day.After intervention,the mice were killed and the changes of lipid content and activity in adipose tissue were detected by histopathology and immunohistochemistry.The effects of irisin at different concentrations(0,20 and 40 nmol/L)on primary white adipocytes and brown adipocytes were evaluated by immunohistochemistry on uncoupling protein 1(UCP1).In order to further evaluate whether irisin has the function of improving metabolism,the changes of serum indexes and hepatic steatosis in mice fed with high-fat diet were monitored.Results The primary white and brown adipocytes derived from mice were successfully cultured and identified in vitro.In NCD mice,the weight gain of mice with irisin was lower than that of control mice[(-0.78±0.98)vs(0.27±0.55)g].Histopathology showed that the area of white adipocytes with irisin was smaller than controls[(14.78±8.44)vs(29.49±12.97)μm2]and the brown adipocytes were larger than controls[(0.92±0.35)vs(0.19±0.12)μm2](both P<0.05),while the expression of UCP1 in both adipose tissues was significantly higher in irisin group.After irisin treatment,the levels of blood glucose[(7.18±0.41)vs(13.48±2.07)mmol/L,P<0.01]and cholesterol[(2.38±0.26)vs(3.89±0.93)mmol/L,P<0.05]were significantly lower than controls,and the content of lipid droplets in liver cells was less than controls[(2.73±1.96)%vs(14.04±6.29)%,P<0.001].Conclusions Low dose irisin can promote the br
作者
陈月
赵宇飞
江洋
吴红红
居胜红
彭新桂
Chen Yue;Zhao Yufei;Jiang Yang;Wu Honghong;Ju Shenghong;Peng Xingui(Department of Radiology,Zhongda Hospital(Jiangsu Key Laboratory of Molecular and Functional Imaging),Nanjing 210009,China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2021年第16期1165-1170,共6页
National Medical Journal of China
基金
国家自然科学基金 (81871412,81501523)。
