摘要
为了获得高均匀性的叶绿素荧光主动诱导激发光源,获得更有对比性的叶绿素荧光参数,针对不同高度、不同距离下样品区域的辐照度分布,提出以样本范围内辐照度最小值与辐照度平均值之比做激发光均匀性指标。通过对不同个数组成的三种基础LED阵列A0,A1,A2进行空间模拟仿真,结果表明,辐照度均匀性与LED阵列高度和LED间距呈正相关,辐照均匀度随LED数目的增加而增加。在0.04m^(2)的研究区域内LED间距0.04m、阵列高度0.5m时,A2阵列辐照度均匀度最大,约为0.920。利用手持式照度计验证了LED阵列激发光分布均匀性,实验结果与仿真结果一致。通过CCD相机采集荧光图像,分析了不同激发阵列排布下叶片荧光分布,为今后高均匀性激发光下叶片区域发射荧光差异分析提供了思路。
In order to obtain a high uniformity of chlorophyll fluorescence active induced excitation source and more contrasting chlorophyll fluorescence parameters,the ratio of the minimum irradiance to the mean irradiance in the sample area was proposed as the index of excitation uniformity for the irradiance distribution of the sample area under different numbers of light sources and at different distances.Three kinds of basic LED arrays A0,A1 and A2 were simulated by spatial simulation.The results showed that the irradiance uniformity was positively correlated with the LED array height and LED spacing,and the irradiance uniformity increases with the increase of the number of LEDs.In the research area of 0.04 m^(2),when the LED spacing is 0.04 m and the array height is 0.5 m,the irradiance uniformity of A2 array is the largest,which is about 0.920.The uniformity of excitation light distribution of LED arrays was verified using a handheld illuminance meter,and the experimental results were consistent with the simulation results.The fluorescence images were collected by CCD camera,and the leaf fluorescence distribution under different excitation array arrangement was analyzed,which provides an idea for future analysis of the difference of leaf regional emission fluorescence under high uniformity excitation light.
作者
马祯元
景敏
陈曼龙
杨帆
丁敏
张琦
MA Zhenyuan;JING Min;CHEN Manlong;YANG Fan;DING Min;ZHANG Qi(School of Mechanical Engineering,Shaanxi University of Technology,Hanzhong 723000,China;Key Laboratory of Industrial Automation in Shaanxi Province,Hanzhong 723000,China)
出处
《光学技术》
CAS
CSCD
北大核心
2021年第2期129-134,共6页
Optical Technique
基金
陕西省科技厅项目(2019JM-171)
陕西省教育厅专项(18JK0163)
陕西省教育厅专项(18JK0145)。
关键词
LED激发阵列
模拟分析
主动诱导叶绿素荧光
数字图像处理
LED excitation arrays
simulation analysis
active induction of chlorophyll fluorescence
digital image processing
