摘要
建立鸡肉和鸡蛋中阿比多尔、金刚烷胺、金刚乙胺、美金刚、奥司他韦的在线固相萃取-液相色谱-串联质谱检测方法。样品采用1.0%甲酸-乙腈提取,经MCX在线固相萃取小柱净化,用XBridge C_(18)色谱柱分离,使用电喷雾离子源正离子模式监测,以内标法定量。结果表明:5种抗病毒类药物在0.05~100 ng/mL范围内线性关系良好;检出限为0.05~0.10μg/kg,定量限为0.10~0.20μg/kg;方法回收率范围为80.03%~93.96%,相对标准偏差低于10%。该方法分析速度快、灵敏度高,可用于鸡肉和鸡蛋中5种抗病毒类药物的定性、定量分析。
In this research,we developed an on-line solid phase extraction(SPE)combined with liquid chromatographytandem mass spectrometry(HPLC-MS/MS)method for the detection of arbidol,amantadine,rimantadine,memantine and oseltamivir in chicken meat and eggs.Samples were extracted with 1.0%formic acid in acetonitrile,and the extract was purified using an MCX on-line solid phase extraction cartridge.The target substances were separated using an XBridge C_(18) column,detected by electrospray ionization in the positive ion mode,and quantitated by the internal standard method.The results showed that the calibration curves of all analytes had a good linear relationship in the concentration range of 0.05–100 ng/mL.The limit of detection(LOD)and the limit of quantitation(LOQ)were 0.05–0.10μg/kg and 0.10–0.20μg/kg,respectively.The recoveries of the developed method were in the range of 80.03%–93.96%,with relative standard deviations(RSDs)of lower than 10%.The method was proven to be simple and sensitive,and could be used for the qualification and quantification of the five antiviral drugs in chicken meat and eggs.
作者
马俊美
何亮娜
孙磊
王东
张雷雷
张岩
康文艺
MA Junmei;HE Liangna;SUN Lei;WANG Dong;ZHANG Leilei;ZHANG Yan;KANG Wenyi(Hebei Key Laboratory of Forensic Medicine,College of Forensic Medicine,Hebei Medical University,Shijiazhuang 050017,China;Hebei Food Safety Key Laboratory,Hebei Food Inspection and Research Institute,Shijiazhuang 050227,China;National R&D Center for Edible Fungus Processing Technology,Henan University,Kaifeng 475004,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2021年第10期305-310,共6页
Food Science
基金
“十三五”国家重点研发计划重点专项(2018YFC1603400)
河北省高层次人才资助项目(A2017005008)。
关键词
在线固相萃取
液相色谱-串联质谱法
抗病毒类药物
内标法定量
on-line solid phase extraction
liquid chromatography-tandem mass spectrometry
antiviral drugs
internal standard quantification
