摘要
建立了鸡肉组织中金刚烷胺残留检测的超高效液相-串联质谱方法。采用三氯乙酸∶乙腈(1∶1,V/V)提取试样中残留的金刚烷胺,SPE净化浓缩,ACQUITY UPLCTMBEH C18色谱柱为分离柱,正离子扫描上机测定。结果显示,金刚烷胺在1～20 ng/mL的范围内线性关系良好,R2=0.997;样品检出限为1 ng/g,定量限为2 ng/g。在2、5、10 ng/g添加水平的回收率为85%～125%,批内批间变异系数均小于20%。本方法快速、灵敏、重现性好,适用于鸡肉中金刚烷胺的残留检测。
A mothod was established for determination of amantadine residues in chicken tissue by UPLC - MS/MS. Samples were extracted by acetocaustin - acetonitrile （ 1 ： 1, V/V）. Sample purification and concentration were performed by SPE with watersoasis mcx column. Then the reconstituted sample solution was analyzed by UPLC-MS/MS under positive ion mode, with ACQUITY UPLCTMBEH C18 as the analytical column. The results showed that the good linear range was 1.0 -20 ng/mL（R^2 = 0.997）. The detection limit was 1 ng/g, and the quantitation limit was 2 ng/g. The recoveries ranged from 85% - 125% and the relative standard deviations were below 20% following analysis of spiked at concentrations 2, 5, 10 ng/g. The mothod was reliable, sensitive and reproducibility, adapts to the determination of amantadine residues in chicken tissue.
出处
《中国兽药杂志》
2013年第6期53-55,共3页
Chinese Journal of Veterinary Drug
