摘要
建立了公英青蓝合剂中非法添加金刚乙胺检测的UPLC-MS/MS方法。样品经甲醇提取稀释后,采用ACQUITY UPLC~® BEH C_(18)色谱柱为分离柱,质谱正离子扫描分析测定。结果显示,金刚乙胺在0.5～50 ng/mL范围内线性关系良好(R^2=0.9985);样品检出限为2μg/mL,定量限为5μg/mL;在5、25、50、100μg/mL添加水平的回收率为90%～110%,批内批间变异系数均小于10%。本方法灵敏、快速、重现性好,适用于公英青蓝合剂中非法添加金刚乙胺的检测。
A method was established for determination of rimantadine added in Gongying Qinglan Mixture by UPLC-MS/MS. The sample was extracted and diluted with methanol, the ACQUITY UPLCR BEH C18 was used as the separation column, and the determination was conducted by scanning positive ion by mass spectrometry. The results showed that the linear relationship of rimantadine was better in the range of 0.5 to 50 ng/mL(R^2=0.9985).The detection limit was 2 μg/mL, and the quantitation limit was 5 μg/mL. The analysis of spiked 5, 25, 50, 100 μg/mL rimantadine showed that the recoveries ranged from 90% to 110% and the relative standard deviations were below 10%. The experiment showed that the method was reliable, sensitive and reproducible and suitable for the determination of rimantadine illegally added in Gongying Qinglan Mixture.
作者
魏秀丽
张志民
赵有轩
张传津
李有志
Wei Xiuli;Zhang Zhimin;Zhao Youxuan;Zhang Chuanjin;Li Youzhi(Institute of Veterinary Drug Quality Inspection of Shandong Province/Shandong Provincial Key Laboratory of Quality Safety Monitoring and Risk Assessment for Animal Products,Jinan 250022,China)
出处
《山东农业科学》
2019年第3期124-127,共4页
Shandong Agricultural Sciences
基金
国家科技支撑计划项目"兽药安全与质量评价技术研究与应用"(2015BAD11B03-01)
