摘要
目的研究异氟醚对前列腺癌细胞DU-145增殖和凋亡的影响,并探究对TLR4信号通路的影响。方法分别采用0%、1%和2%的异氟醚处理DU-145细胞6 h,设为对照组、低剂量组和高剂量组,采用MTT检测各组细胞的增殖能力,采用流式细胞仪检测各组细胞的凋亡水平,采用ELISA试剂盒检测各组细胞中炎症因子的含量,采用qPCR检测各组细胞中TLR4信号通路相关mRNA的表达水平,采用Western检测凋亡相关蛋白和TLR4信号通路相关蛋白的表达水平。结果与对照组比较,低剂量组和高剂量组细胞的增殖能力显著增加(P<0.01),凋亡水平显著降低(P<0.01),凋亡相关蛋白Caspase 3的表达显著降低(P<0.01),Bcl-2/Bax显著增加(P<0.01),炎症因子IL-6和TNF-α的含量显著降低(P<0.01),IL-10的含量显著增加(P<0.01),TLR4、MyD88和NF-κB蛋白和mRNA的表达水平均显著增加(P<0.01),以上差异均有统计学意义。结论异氟醚可显著增加前列腺癌DU-145的增殖能力,降低细胞的凋亡水平,其机制可能与激活TLR4信号通路相关。
Objective To studied the effect of Isoflurane on proliferation and apoptosis of prostate cancer cell DU-145 through TLR4 pathway.Methods DU-145 cells were treated with 0%,1%,and 2%isoflurane for 6 hours,respectively,as control group,low-dose group and high-dose group.MTT was used to detect the proliferation of cells in each group.Flow cytometry wasused to detect theapoptosis of cells in each group.ELISA kit was used to detect the content of inflammatory factors in each group of cells.qPCR was used to analyze the mRNA expression of TLR4 pathway.Western blot was used to detect the protein expression of TLR4 pathway.Results Compared with control group,the cell proliferation of low-dose group and high-dose group was significantly increased(P<0.01),the level of apoptosis was significantly decreased(P<0.01),the protein expression of Caspase 3 was significantly decreased(P<0.01),Bcl-2/Bax was increased(P<0.01),the contents of IL-6 and TNF-were decreased(P<0.01),the content of IL-10 was increased(P<0.01),the mRNA and protein expression of TLR4,MyD88 and NF-κB were significantly increased(P<0.01),above differences are statistically significant.Conclusion Isoflurane can significantly increase the proliferation and decreased the level of apoptosis of prostate cancer DU-145,the mechanism may be related to the activation of TLR4 pathway.
作者
席文娟
么安亮
李军
蔡立松
XI Wenjuan;ME Anliang;LI Jun;CAI Lisong(Department of Anesthesiology,Kailuan General Hospital of Tangshan,Tangshan 063000,China;不详)
出处
《中国煤炭工业医学杂志》
2021年第2期118-123,共6页
Chinese Journal of Coal Industry Medicine
基金
2017年度河北省医学科学研究重点课题(编号:20170907)。
