摘要
目的观察沉默前列腺跨膜雄激素诱导蛋白1(PMEPA1)对肝癌细胞HepG2增殖和迁移的影响。方法应用qRT-PCR、免疫组化染色和Western blot方法检测PMEPA1在肝癌细胞HepG2中mRNA和蛋白表达情况。分析PMEPA1在肝细胞性肝癌(HCC)组织中的表达及其与临床病理特征的关系。使用siRNA干扰肝癌细胞HepG2中PMEPA1的表达,并应用MTT法,Transwell法检测肝癌细胞HepG2的增殖及迁移能力。结果PMEPA1蛋白在HCC组织中表达高于正常肝组织。肝癌组织内PMEPA1蛋白表达与HCC患者肿瘤大小(P=0.0366)和淋巴结转移(P=0.0358)密切相关。与HCC患者性别、年龄、AFP水平高低、脉管浸润、组织学分级及TNM分期等均无相关性。PMEPA1-siRNA肝癌细胞HepG2 PMEPA1蛋白及mRNA表达均下调(P<0.01)。siPMEPA1转染细胞增殖能力及迁移能力均降低(P<0.01)。结论PMEPA1表达可能促进HCC的增殖和迁移。
Objective To observe the effect of silencing prostate transmembrane protein, androgen induced 1(PMEPA1) on proliferation and migration of HepG2 hepatocellular carcinoma(HCC) cells. Methods qRT-PCR, immunohistochemistry, and Western blot were used to detect the mRNA and protein expression of PMEPA1 in HepG2 HCC cells. Besides, the relationship between the expression of PMEPA1 in liver cancer and its clinicopathological features was analyzed. Additionally, siRNA was employed to interfere with the expression of PMEPA1 in HepG2 HCC cells. MTT and Transwell methods were adopted to detect the proliferation and migration of HepG2 HCC cells. Results The expression of PMEPA1 protein in liver cancer was higher than that in normal liver, which was correlated with tumour size(P=0.036 6) and lymph node metastasis(P=0.035 8). Meanwhile, it had no correlation with gender, age, AFP level, vascular infiltration, histological grade, and TNM stage of HCC patients. Moreover, the PMEPA1 protein and mRNA expression of PMEPA1-siRNA HepG2 HCC cells was down-regulated(P<0.01), and the proliferation and migration of transfected cells in siPMEPA1 both decreased(P<0.01). Conclusion The expression of PMEPA1 may promote the proliferation and migration of hepatocellular carcinoma cells.
作者
张涛
陈强
杜卫东
吴正升
Zhang Tao;Chen Qiang;Du Weidong(Dept of Pathology,The First Affiliated Hospital of Anhui Medical University,Hefei 230032;Dept of Pathology,Anhui Medical University,Hefei 230032)
出处
《安徽医科大学学报》
CAS
北大核心
2021年第5期741-745,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81472493、81972472)
安徽省转化医学研究院科研基金项目(编号:2017zhyx37)。
关键词
肝细胞癌
PMEPA1
免疫组化
细胞增殖
转移
hepatocellular carcinoma
PMEPA1
immunohistochemistry
proliferation
migration
