摘要
背景慢性阻塞性肺疾病(COPD)发病率高且严重影响患者的生活质量,益气固表丸治疗COPD患者具有较好的疗效,但其具体作用机制目前尚未明确。目的探讨基于核转录因子κB(NF-κB)信号通路的益气固表丸治疗COPD的疗效,以期为益气固表丸治疗COPD提供科学依据。方法本实验时间为2017年7月—2018年6月,选取鼠龄为8周的SPF级雄性SD大鼠48只,按照随机数字表法分为空白对照组、模型组、益气固表丸高剂量组、益气固表丸低剂量组,每组12只。适应性喂养1周后,除空白对照组外的大鼠均建立COPD模型。益气固表丸高剂量组给予益气固表丸200丸进行治疗,益气固表丸低剂量组给予益气固表丸100丸进行治疗,持续治疗14 d;空白对照组和模型组大鼠使用相同剂量的0.9%氯化钠溶液和灌胃频率处理。观察大鼠肺组织病理变化情况。比较四组大鼠血清肿瘤坏死因子α(TNF-α)、白介素(IL)-6水平和RelA(p65)、NF-κB1(p53)基因相对表达量及IκBα、p-IκBα蛋白相对表达量。结果(1)HE染色结果显示,空白对照组大鼠肺组织大体结构基本正常;模型组大鼠肺组织肺泡壁增生、增厚,伴细胞增大、增多,间质较多慢性炎症细胞浸润,部分肺泡萎缩,部分肺泡扩张,支气管上皮细胞部分增生及缺损,黏膜下大量慢性炎症细胞浸润,较多出血、坏死区;与模型组相比,益气固表丸低剂量组大鼠肺组织病变改善不明显;与模型组相比,益气固表丸高剂量组大鼠肺泡增生、增厚及间质炎症细胞浸润程度均有所改善,肺泡萎缩及肺泡壁断裂、扩张程度均减轻,支气管病变有所好转。(2)模型组、益气固表丸低剂量组大鼠血清TNF-α、IL-6水平及益气固表丸高剂量组血清TNF-α水平高于空白对照组(P<0.05);益气固表丸高剂量组大鼠血清TNF-α、IL-6水平低于模型组及益气固表丸低剂量组(P<0.05)。(3)模型组、益气固表丸低剂量组大鼠RelA(p65
Background The incidence of chronic obstructive pulmonary disease(COPD)is high and seriously impact quality of life of patients.Yiqigubiao pill has a good therapeutic effect,but the specific mechanism is not clear at present.Objective To explore the effect of Yiqigubiao pill on COPD based on nuclear transcription factorκB(NF-κB)signal pathway,in order to provide scientific basis for treating COPD with Yiqigubiao pill.Methods The experiment time was from July 2017 to June 2018,48 SPF male SD rats aged 8 weeks were selected and divided into blank control group,model group,high-dose group and low-dose group according to random number table method,12 rats in each group.After adapted feeding for 1 week,all rats was established COPD model except the rats in blank control group.The high-dose group was treated with Yiqigubiao pill 200 pills,while the low-dose group was treated with Yiqigubiao pill 100 pills,both groups were treated for 14 days;and rats in the blank control group and the model group were treated with the same dose of 0.9%sodium chloride solution and gastric perfusion frequency.The pathological changes of lung tissue in rats were observed.Serum tumor necrosis factor alpha(TNF-α),interleukin(IL)-6 levels and relative expression of RelA(p65),NF-κB1(p53)gene and IκBα,p-IκBαprotein were compared in the four groups.Results(1)Hematoxylin-eosin staining showed that,the general structure of lung tissue in blank control group was basically normal;in model group,the alveolar wall of lung tissue proliferated and thickened,accompanied by enlargement and increase of cells,infiltration of chronic inflammatory cells in interstitial tissue,atrophy of partial alveoli,expansion of partial alveoli,partial proliferation and defect of bronchial epithelial cells,infiltration of a large number of chronic inflammatory cells under mucosa,and more bleeding and necrosis areas.Compared with model group,the low-dose group had no significant difference in pathological changes,and the alveolar hyperplasia,thickening and intersti
作者
罗建江
张艳丽
刘珊
LUO Jianjiang;ZHANG Yanli;LIU Shan(Department of Respiration,Institute of Traditional Chinese Medicine of Xinjiang Uygur Autonomous Region,Urumqi 830000,China;Xinjiang Medical University,Urumqi 830000,China)
出处
《实用心脑肺血管病杂志》
2021年第6期87-92,98,共7页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
基金
新疆维吾尔自治区自然科学基金资助项目(2017D01C169)。
