摘要
在45℃控制劣变条件下处理同德老芒麦(Elymus sibiricus cv.Tongde)种子60d,获得不同活力水平的种子样品(D0、D26、D30、D40、D60),分析比较种子活力变化规律。结果表明:随着劣变程度的加深,老芒麦种子发芽势、发芽率、发芽指数和活力指数逐渐下降。劣变种子(D0、D30、D40、D60)的种胚线粒体生理特性显示:种胚线粒体苹果酸脱氢酶(MDH)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性逐渐降低,单脱氢抗坏血酸还原酶(MDHAR)活性逐渐升高,但在D60处理中超氧化物歧化酶(SOD)活性最高。在老芒麦种子劣变过程中,种胚线粒体超氧阴离子(O_(2)^(-)·)产生速率逐渐升高,过氧化氢(H_(2)O_(2))含量逐渐降低,而SOD和APX协同响应,抗坏血酸-谷胱甘肽循环系统(AsA-GSH)承担清除活性氧(ROS)的作用。
Elymus sibiricus cv.Tongde seeds were treated at 45℃under controlled deterioration conditions for 60 days to obtain samples(D0,D26,D30,D40,D60)with different vigor levels,in which the seed vigor changes were analyzed.The results showed that the germination potential,germination percentage,germination index and vigor index of seeds were decreased with the exacerbation of deterioration.The physiological characteristics of embryo mitochondria of deteriorating seeds(D0,D30,D40,D60)exhibited that the activities of embryonic mitochondria malate dehydrogenase(MDH),ascorbate peroxidase(APX)and glutathione reductase(GR)gradually decreased,and the activity of monodehydroascorbate reductase(MDHAR)gradually increased,but superoxide dismutase(SOD)activity was the highest in D60 treatment.The production rate of embryonic mitochondrial superoxide anion(O_(2)^(-)·)gradually increased and the content of hydrogen peroxide(H_(2)O_(2))gradually decreased during the deterioration process.While SOD and APX responded synergistically,the ascorbic acid-glutathione cycle system(AsA-GSH)was responsible for scavenging reactive oxygen species(ROS).
作者
唐嘉
毛培胜
毛春力
黄琪
赵美琦
TANG Jia;MAO Pei-sheng;MAO Chun-li;HUANG Qi;ZHAO Mei-qi(College of Grassland Science and Technology,China Agricultural University/Key Laboratory of Pratacultural Science,Beijing Municipality,Beijing 100193,China)
出处
《中国草地学报》
CSCD
北大核心
2021年第5期1-7,共7页
Chinese Journal of Grassland
基金
现代农业产业技术体系建设专项资金(CARS-34)
草业科学北京重点实验室资助。
关键词
老芒麦
控制劣变
种子活力
种胚线粒体
抗氧化生理
Elymus sibiricus
Controlled deterioration
Seed vigor
Seed embryonic mitochondria
Antioxidation
