摘要
目的探讨MTH1抑制剂TH588对人恶性黑色素瘤A375细胞增殖、迁移的影响。方法利用细胞培养手段培养人恶性黑色素瘤A375细胞;应用倒置显微镜法观察TH588对细胞形态的影响;采用CCK-8法检测不同浓度TH588(0、4、8、16、32、64μmol/L)对A375细胞的增殖抑制作用;采用集落克隆形成抑制试验观察对比高中低三种浓度(12.8、6.4、3.2μmol/L)TH588对A375细胞的增殖抑制作用;细胞划痕实验检验TH588对A375细胞迁移能力的影响。结果CCK-8检测结果显示,同一时间段不同浓度TH588组与空白对照组比较,细胞存活率均降低;32、64μmol/L TH588浓度下培养48、72 h时细胞存活率均低于同TH588浓度下培养24 h时。倒置显微镜法观察TH588作用于人恶性黑色素瘤A375细胞后,细胞明显皱缩,且边缘变得模糊,有碎片颗粒产生。集落克隆形成抑制实验表明,TH588对人恶性黑色素瘤A375细胞增殖有明显抑制作用。细胞划痕实验表明,经TH588作用后A375细胞划痕迁移距离在48 h内均较无干预的A375细胞迁移距离更短。结论TH588对人恶性黑色素瘤A375细胞的增殖及迁移有明显抑制作用,且低浓度的TH588便有较好的抑制效果。
Objective To investigate the effect of MTH1 inhibitor TH588 on the proliferation and migration of human malignant melanoma A375 cells.Methods Cell culture method was used to culture human malignant melanoma A375 cells,the inverted microscope method was used to observe the influence of TH588 on cell morphology,and the CCK-8 method was used to detect the inhibitory effect of different concentrations of TH588(0,4,8,16,32,64μmol/L)on the proliferation of A375 cells.The colony cloning inhibition test was used to observe and compare the proliferation inhibition effect of TH588 on A375 cells at three concentrations(12.8,6.4 and 3.2μmol/L),cell scratch experiment was used to detect the effect of TH588 on the migration ability of A375 cells.Results The CCK-8 test results showed that the cell survival rate of the TH588 groups with different concentrations at the same time period was lower than that of the blank control group.The cell survival rate at the concentration of 32 and 64μmol/L TH588 at 48 and 72 h was lower than that at 24 h.The results of the inverted microscope method showed that after TH588 acted on human malignant melanoma A375 cells,the cells shrank significantly,the edges became blurred,and debris particles were produced.Colony cloning inhibition test showed that TH588 had a significant inhibitory effect on the proliferation of human malignant melanoma A375 cells.The cell scratch experiment showed that the scratch migration distance of A375 cells after TH588 was shorter than that of A375 cells without intervention within 48 h.Conclusion TH588 has a significant inhibitory effect on the proliferation and migration of human malignant melanoma A375 cells,and low concentration TH588 has a better inhibitory effect.
作者
霍继武
李光早
李璐
熊竹友
李薇
HUO Ji-wu;LI Guang-zao;LI Lu;XIONG Zhu-you;LI Wei(Department of Burn Plastic Surgery,the First Affiliated Hospital of Bengbu Medical College,Anhui Province,Bengbu 233000,China;Pharmacy Intravenous Admixture Service,the First Affiliated Hospital of Bengbu Medical College,Anhui Province,Bengbu 233000,China)
出处
《中国当代医药》
CAS
2021年第15期77-80,共4页
China Modern Medicine
