摘要
目的:观察长期离心运动和针刺干预后大鼠骨骼肌纤维化的情况及转化生长因子β1(TGF-β1)/细胞外信号调节激酶(ERK)/结缔组织生长因子(CTGF)通路的变化,探讨针刺干预在运动导致骨骼肌纤维化中的作用及其机制。方法:SD大鼠随机分为正常组、运动组和针刺组,每组10只。运动组和针刺组大鼠进行3周离心跑台运动,建立大鼠骨骼肌纤维化模型;针刺组在每次运动结束后即刻用针灸针从远端沿小腿三头肌纵向斜刺,穿过小腿三头肌肌腹,留针2 min。扫描电镜观察各组大鼠骨骼肌胶原纤维沉积情况,Western blot法检测各组大鼠腓肠肌Ⅰ型胶原蛋白(CollagenⅠ)、TGF-β1、ERK、磷酸化(p)ERK、CTGF蛋白表达变化。结果:与正常组比较,运动组大鼠骨骼肌胶原纤维出现明显的增生和沉积;针刺组胶原纤维增生程度明显轻于运动组。与正常组比较,运动组大鼠腓肠肌CollagenⅠ、TGF-β1、CTGF蛋白表达及p-ERK/ERK明显升高(P<0.01,P<0.05);与运动组比较,针刺组大鼠腓肠肌CollagenⅠ、TGF-β1、CTGF蛋白表达显著降低(P<0.05,P<0.01)。结论:慢性运动损伤可使骨骼肌胶原纤维沉积,骨骼肌纤维化,针刺可在一定程度上抑制骨骼肌纤维化,可能与下调TGF-β1/ERK/CTGF通路有关。
Objective To observe the changes of the skeletal muscle fibrosis and changes of transforming growth factor-β1(TGF-β1)/extracellular signal-regulated kinase(ERK)/connective tissue growth factor(CTGF)pathway in rats after long-term eccentric exercise and acupuncture intervention, so as to explore the mechanism of acupuncture in regulating exercise-induced skeletal muscle fibrosis. Methods A total of 30 male SD rats were randomly divided into normal control, exercise and acupuncture group, with 10 rats in each group. The rat model of skeletal muscle fibrosis was established by eccentric exercise for 3 weeks. After exercise trained every time, the rats of the acupuncture group received acupuncture stimulation by holding the acupuncture needle to obliquely and longitudinally penetrate the ventral part of triceps of the lower leg along its lateral side, followed by retaining the needle for 2 min. Changes of the collagen fibers in each group was observed by scanning electron microscope. The expressions of Collagen Ⅰ, TGF-β1, phosphated(p)-ERK/ERK and CTGF proteins were detected by Western blot. Results After 3 weeks of eccentric exercise and acupuncture, the fibrosis and deposition of collagen fibers in the exercise group were significantly higher than that in the normal control group, the degree of fibrosis in the acupuncture group was significantly lower than that in the exercise group. Compared with the normal control group, the expression levels of Collagen Ⅰ, TGF-β1, CTGF and p-ERK/ERK in the exercise group was significantly higher(P<0.01,P<0.05). After EA interventions, the increased levels of the above indicators were significantly reversed(P<0.05,P<0.01) apart from p-ERK/ERK which had a downward trend, but the difference was not statistically significant. Conclusion The accumulation of chronic sports injury can lead to the deposition of collagen fibers in skeletal muscle, which leads to the fibrosis of skeletal muscle. Acupuncture can inhibit skeletal muscle fibrosis via down-regulating TGF-β1/ERK/CTGF
作者
雷槟恺
赵硕
徐涛
周越
许寿生
王瑞元
李俊平
LEI Bing-kai;ZHAO Shuo;XU Tao;ZHOU Yue;XU Shou-sheng;WANG Rui-yuan;LI Jun-ping(School of Sports Human Science,Beijing Sport University,Beijing 100084,China)
出处
《针刺研究》
CAS
CSCD
北大核心
2021年第4期306-311,共6页
Acupuncture Research
基金
国家自然科学基金项目(No.31471133)
中央高校基本科研业务费专项资金资助课题(No.2019PT013、校2020022、校2020025)。
