摘要
目的:探讨黄芪总皂苷对重症急性胰腺炎大鼠肠道损伤的保护作用及对肠组织p38丝裂原活化蛋白激酶(p38MAPK)/核因子κB (NF-κB)表达的影响。方法:将100只SD大鼠分为对照组、模型组、地塞米松组及黄芪总皂苷低、高剂量组(以下简称低、高剂量组)。除对照组外,其余各组用牛磺胆酸钠诱导重症急性胰腺炎大鼠模型。模型制备成功后,低、高剂量组及地塞米松组给予相应药物腹腔注射,对照组和模型组给予等体积生理盐水。24 h后获取大鼠胰腺、肠黏膜组织及血清,测定黏膜损伤指数、血清淀粉酶、胰腺比重,HE染色观察肠黏膜损伤病理情况,测定肠黏膜p38MAPK、NF-κB蛋白和mRNA表达水平。结果:与对照组比较,模型组肠黏膜损伤指数、血清淀粉酶、胰腺比重、肠组织p38MAPK、NF-кB mRNA和蛋白表达水平升高(P<0.05);与模型组比较,地塞米松组和低、高剂量组以上各指标降低(P<0.05);与地塞米松组比较,低剂量组以上各指标升高(P<0.05);与低剂量组比较,高剂量组以上各指标降低(P<0.05)。结论:黄芪总皂苷对重症急性胰腺炎大鼠肠道损伤有保护作用,可减轻急性胰腺炎大鼠肠道炎症反应;其机制与黄芪总皂苷抑制p38MAPK、NF-κB mRNA和蛋白表达,进而抑制p38MAPK/NF-κB炎症通路的激活有关。
Objective:To investigate the protective effect of total saponins of astragalus on intestinal injury in rats with severe acute pancreatitis and its effect on the expressions of p38 mitogen-activated protein kinase(p38MAPK)/nuclear factorκB(NF-κB) in intestinal tissues. Methods:A total of 100 SD rats were divided into the control group,the model group,the dexamethasone group and the total saponins of astragalus groups with low and high doses respectively(the low-dose group and the high-dose group). Except for the control group,the other groups were given sodium taurocholate to induce the rat models with severe acute pancreatitis. After successful model preparation,the low-dose group,the high-dose group and the dexamethasone group were given intraperitoneal injection with corresponding medicine;the control group and the model group were given the same volume of normal saline. After 24 hours,the pancreatic and intestinal mucosal tissues as well as the serum of rats were obtained to measure the indexes of mucosal injury, the serum amylase and the proportions of pancreas. The pathological conditions of intestinal mucosal injury were observed by HE staining,and the expression levels of p38MAPK,NF-κB proteins and mRNA in intestinal mucosa were measured. Results:Compared with those in the control group, the indexes of intestinal mucosal injury, the serum amylase and the proportion of the pancreas as well as the expression levels of p38MAPK, NF-κB m RNA and protein in the model group were increased(P<0.05);compared with those in the model group,the above indexes in the dexamethasone group,the low-dose group and the high-dose group were decreased(P<0.05);compared with those in the dexamethasone group,the above indexes in the low-dose group were increased(P<0.05);compared with those in the low-dose group,the above indexes in the high-dose group were decreased(P<0.05). Conclusion: Total saponins of astragalus has protective effect on intestinal injury in rats with severe acute pancreatitis,and can relieve intestinal infla
作者
金玲玲
汤斌斌
周晶晶
莫霏霏
JIN Lingling;TANG Binbin;ZHOU Jingjing;MO Feifei
出处
《新中医》
CAS
2021年第7期6-10,共5页
New Chinese Medicine
