摘要
目的:观察筋骨草总黄酮(TFA)含药血清对肾小球系膜细胞(GMC)p38丝裂原激活蛋白激酶/核转录因子-κB(p38MAPK/NF-κB)信号通路的影响,进一步探讨TFA保护肾脏的可能机制。方法:体外培养大鼠GMC,以脂多糖(LPS)诱导大鼠GMC增殖,加入TFA含药血清进行干预。采用ELISA法检测细胞上清液中IL-1β、TNF-α的含量;RT-PCR法检测NF-κB mRNA和IκB m RNA的表达水平;Western Blot法检测p-p38MAPK蛋白的表达情况。结果:TFA含药血清干预24、48h后细胞上清液中IL-1β、TNF-α的含量较LPS组均明显减少(P<0.05,P<0.01)。干预48h后,IκB m RNA的相对表达量较LPS组明显增多,而NF-κB mRNA和p-p38MAPK蛋白的相对表达量均明显减少(P<0.05,P<0.01)。结论:TFA的肾脏保护作用可能与调控p38MAPK/NF-κB信号通路,继而减弱下游炎性因子IL-1β、TNF-α的表达有关。
Objective: To observe the effect of serum containing total flavonoids of Ajuga(TFA) on p38 mitogen activated protein kinase/nuclear factor κB(p38MAPK/NF-κB) signal pathway of glomerular mesangial cells(GMCs), and to explore the possible protective mechanism of TFA on kidney. Methods: Rat GMCs were cultured in vitro. The proliferation of rat GMCs was induced by lipopolysaccharide(LPS), then intervened by the serum containing TFA. The content of interleukin1 beta(IL-1β), tumor necrosis factor α(TNF-α) in the cell supernatant was detected by ELISA method. NF-κB m RNA and inhibitory κB(IκB) m RNA were detected by RT-PCR. p-p38 MAPK protein was detected by Western Blot. Results: After intervention with the serum containing TFA for 24 hours and 48 hours, the supernatant content of IL-1β and TNF-α were significantly lower than those of LPS group(P<0.05, P<0.01). After 48 hours of the intervention, the expression of IκB m RNA increased significantly than those of LPS group, where as the expression of NF-κB m RNA and p-p38 MAPK protein were significantly decreased(P<0.05, P<0.01). Conclusion: The kidney protection of TFA might be related to the regulation of p38MAPK/NF-κB signaling pathway, and then with the low expression of related down stream inflammatory factor such as IL-1β, TNF-α.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2017年第8期3764-3767,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
福建省科技厅自然科学基金项目(No.2014J05094)~~
