摘要
目的采用直接测序法对主要组织相容性复合物Ⅰ类链相关A基因(major histocompatibility complex classichain-related A,MICA)进行分型,了解MICA等位基因在正常人群、急性淋巴细胞白血病(acute lymphocytic leukemia,ALL)、急性髓性白血病(acute myeloid leukemia,AML)、骨髓增生异常综合征(myelodysplastic syndrome,MDS)患者中分布的差异。方法选取2016年北京市红十字血液中心167份白血病患者DNA样本(病例组)和224份正常人样本(正常对照组),对MICA基因2~6外显子进行测序分型,并判读结果。分析MICA等位基因频率,跨膜区GCT重复次数,MICA-129Met/Val在正常对照组和病例组间的差异。结果发现19个MICA等位基因,与正常对照组比较,大多数MICA等位基因在各组间频率的差异无统计学意义。但MDS组的MICA*010:01频率明显低于正常对照组(P<0.01),而MDS组的MICA*019:01频率明显高于正常对照组、ALL组和AML组,差异均有统计学意义(P<0.01)。结论MICA*010:01和MICA*019:01的分布在MDS患者和正常人群间不同,提示这两个等位基因可能与MDS的易感性相关。
Objective To identify the major histocompatibility complex class I chain-related A(MICA)alleles by direct sequencing and analyze the difference of it frequencies in normal controls,acute lymphocytic leukemia(ALL),acute myeloid leukemia(AML)and myelodysplastic syndrome(MDS)patients.Methods The exons 2-6 of MICA gene were sequenced and typed in 167 patients with leukemia and 224 normal subjects(normal control group).The difference of the frequency of MICA alleles,the number of GCT repeats in transmembrane region and mica-129 Met/Val between normal control group and patients were analyzed.Results Nineteen MICA alleles were identified.Compared with the normal control group,the frequencies of most of the MICA alleles had no significant difference among different groups.However,the frequency of MICA*010:01 in MDS group was significantly lower than that in normal control group(P<0.01),while the frequency of MICA*019:01 in MDS group was significantly higher than that in normal control group,ALL group and AML group(P<0.01).Conclusions The distributions of MICA*010:01 and MICA*019:01 are different between the MDS patients and the normal subjects,indicating that these two alleles may be related to the susceptibility of MDS.
作者
刘娜
王东梅
王洁
李冬妹
王中梅
范成艳
单小燕
Liu Na;Wang Dongmei;Wang Jie;Li Dongmei;Wang Zhongmei;Fan Chengyan;Shan Xiaoyan(HLA Lab,Beijing Red Cross Blood Center,Beijing 100088,China)
出处
《北京医学》
CAS
2021年第2期161-164,共4页
Beijing Medical Journal
关键词
主要组织相容性复合物Ⅰ类链相关A基因
多态性
测序
major histocompatibility complex class I chain-related A(MICA)
polymorphism
sequencing
