摘要
目的:研究白及多糖(BSP)对结肠癌CT26荷瘤小鼠的抗肿瘤及免疫调节作用。方法:从30只BALB/c小鼠中随机选取6只作为对照组(Control),剩余24只小鼠右前肢皮下接种CT26细胞构建CT26结肠癌小鼠模型,建模成功后将模型裸鼠分为荷瘤模型组(Model)、白及多糖低剂量组[BSP-L,100 mg/(kg·d)]、白及多糖高剂量组[BSP-H,400 mg/(kg·d)]和阳性药物香菇多糖组[LNT,150 mg/(kg·d)],每组6只。所有小鼠连续灌胃给药3周,其中对照组和模型组每天给予与药物组等量生理盐水灌胃。比较各组肿瘤体积、肿瘤抑制率及免疫器官指数。MTT法检测脾脏淋巴细胞增殖活性,ELISA法检测血清免疫因子IL-2和IFN-γ水平;流式细胞术检测外周血T淋巴细胞亚群;Western blot检测各组小鼠脾脏组织中TLR4、My D88、NF-κB p65蛋白表达水平。结果:与Model组相比,BSP-H组和LNT组移植瘤体积显著缩小,肿瘤重量显著降低,抑瘤率显著增加(P<0.01)。与Control组相比,Model组小鼠脾脏指数、胸腺指数、脾脏淋巴细胞活性、血清中免疫因子IL-2和IFN-γ水平、外周血CD4^(+)/CD8^(+)及脾脏组织中TLR4、MyD88、NF-κB p65蛋白表达水平显著降低(P<0.05);与Model组相比,BSP-H组小鼠脾脏指数、胸腺指数、脾脏淋巴细胞活性、血清中免疫因子IL-2和IFN-γ水平、外周血CD4^(+)/CD8^(+)及脾脏组织中TLR4、MyD88、NF-κB p65蛋白表达水平显著上升(P<0.05);而BSP-L组与Model组相比,以上各指标变化均无显著性差异(P>0.05)。结论:白及多糖对结肠癌CT26荷瘤小鼠肿瘤生长有抑制作用,可能与增强荷瘤小鼠抗肿瘤免疫、激活TLR4/NF-κB信号通路有关。
Objective:To study the anti-tumor and immunomodulatory effects of Bletilla Striata polysaccharide(BSP)on CT26 tumor-bearing mice.Methods:6 BALB/c mice were randomly selected from 30 BALB/c mice as Control group,and the remaining 24 mice were subcutaneously inoculated with CT26 cells in the right forelimb as CT26 colon cancer mouse model.The 24 tumor bearing nude mice were equally divided into tumor-bearing model group(Model),low-dose BSP group[BSP-L,100 mg/(kg·d)],high-dose BSP group[BSP-H,400 mg/(kg·d)]and positive group[Lentinan,LNT,150 mg/(kg·d)].All mice were given intragastrically for 3 weeks,and the control group and model group were given the same amount of saline every day.The tumor volume,tumor inhibition rate and immune organ index were calculated.Proliferation activity of spleen lymphocytes was detected by MTT assay;serum levels of the immune factors like IL-2 and IFN-γwere detected by ELISA;T lymphocyte subsets in peripheral blood were detected by flow cytometry;expression levels of TLR-4,MyD88,and NF-κB p65 were detected by Western blot.Results:Compared with Model group,volumes of transplanted tumor of BSP-H group and LNT group were significantly reduced,weights of tumors were significantly reduced,and the tumor inhibition rate increased significantly(P<0.01).Compared with Control group,the spleen index,thymus index,spleen lymphocyte activity,serum levels of immune factors IL-2 and IFN-γ,peripheral blood CD4^(+)/CD8^(+),and TLR4,MyD88,NF-κB p65 protein expression levels in spleen tissues of Model group were significantly decreased(P<0.05).Compared with Model group,the spleen index,thymus index,spleen lymphocyte activity,serum levels of immune factors IL-2 and IFN-γ,peripheral blood CD4^(+)/CD8^(+),and TLR4,MyD88,NF-κB p65 protein expression levels in spleen tissues of BSP-H group were significantly increased(P<0.05).However,there was no significant difference in changes of the above indicators between BSP-L group and Model group(P>0.05).Conclusion:Bletilla Striata polysaccharide can inhibit
作者
刘文立
莫海云
LIU Wen-Li;MO Hai-Yun(Oncology Department,Nanhua Hospital Affiliated to Nanhua University,Hengyang 421002,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2021年第8期941-945,共5页
Chinese Journal of Immunology
