摘要
目的建立HPLC法同时测定利胆片中绿原酸、芒果苷、芍药苷、黄芩苷、木香烃内酯、去氢木香内酯6个成分的含量。方法选用Thermo C 18色谱柱(250 mm×4.6 mm,5μm);以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱;流速1 mL·min^(-1),柱温30℃,检测波长315 nm(绿原酸、芒果苷、黄芩苷)、230 nm(芍药苷、木香烃内酯、去氢木香内酯)。结果6个成分分离度良好,绿原酸、芒果苷、芍药苷、黄芩苷、木香烃内酯、去氢木香内酯进样量分别在0.0154~1.5446μg(r=1.000);0.0032~0.3176μg(r=0.9999);0.0258~1.8753μg(r=0.9991);0.0065~0.6517μg(r=0.9996);0.0180~4.4929μg(r=0.9999);0.0188~4.70μg(r=0.9995)范围内与峰面积呈良好的线性关系,平均加样回收率(n=9)为96.47%~102.17%,RSD为1.01~1.93%。结论经方法学验证,本法可用于利胆片中的绿原酸、芒果苷、芍药苷、黄芩苷、木香烃内酯、去氢木香内酯含量测定。
OBJECTIVE To establish an HPLC method for simultaneous determination of chlorogenic acid,chimonin,peoniflorin,baicalin,costunolide and dehydrocostus lactone in LiDan tablets.METHODS The Thermo C 18 column(250 mm×4.6 mm,5μm)was used with the mobile phase of acetonitrile(A)0.1%phosphoric acid solution(B)in a gradient mode at 30℃.The flow rate was 1 mL·min^(-1)and the detection wavelengths were set at 315 nm for chlorogenic acid,chimonin and baicalin,230 nm for peoniflorin,costunolide and dehydrocostus lactone.RESULTS 6 selected components were separated satisfactorily.The linear ranges of chlorogenic acid,chimonin,peoniflorin,baicalin,costunolide and dehydrocostus lactone were 0.0154-1.5446μg(r=1.000);0.0032-0.3176μg(r=0.9999);0.0258-1.8753μg(r=0.9991);0.0065-0.6517μg(r=0.9996);0.0180-4.4929μg(r=0.9999);0.0188-4.70μg(r=0.9995),respectively.The average recoveries(n=9)were in the range of 96.47%-102.17%with the RSD of 1.01%-1.93%.CONCLUSION Proved by method validation,the method could be used to determine contents of chlorogenic acid,chimonin,peoniflorin,baicalin,costunolide and dehydrocostus lactone in LiDan tablets.
作者
周丽清
ZHOU Li-qing(Fujian Institute for Food and Drug Quality Control,Fuzhou 350001,China)
出处
《海峡药学》
2021年第4期81-84,共4页
Strait Pharmaceutical Journal
