摘要
厌氧菌是地球上数量最多、物种最丰富的微生物,也是分类上报道最少的微生物。它们对氧气敏感、生长条件苛刻,不容易培养分离。本文简要总结了厌氧微生物的研究历史,分析了限制厌氧微生物培养分离的主要因素,讨论了厌氧微生物培养分离的策略和方法,回顾了国内外厌氧微生物的系统分类学现状,并展望了厌氧微生物培养分离的发展趋势。
Anaerobes are the largest number of microorganisms on earth but rarely reported from the point view of taxonomy.Owing to oxygen-sensitive and slow-growth,cultivation and isolation of fastidious anaerobe faces a great challenge.In this review,we introduce the research history of anaerobes,and analyze the factors affecting the isolating efficiency of anaerobes.Furthermore,we discuss the current status of isolation and cultivation methods and strategies,and summarize the research progress on systematic taxonomy of anaerobes.In the end,we give an outlook for isolating and cultivating anaerobes.
作者
承磊
马诗淳
巫可佳
张辉
邓宇
Lei Cheng;Shichun Ma;Kejia Wu;Hui Zhang;Yu Deng(Key Laboratory of Development and Application of Rural Renewable Energy,Ministry of Agriculture and Rural Affairs,Biogas Institute of Ministry of Agriculture and Rural Affairs,Chengdu 610041,Sichuan Province,China;China Collection of Anaerobic Microorganisms,Chengdu 610041,Sichuan Province,China;National Agricultural Experimental Station for Microorganisms,Shuangliu,Chengdu 610213,Sichuan Province,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2021年第4期946-968,共23页
Acta Microbiologica Sinica
基金
国家自然科学基金(31570009)
中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-BIOMA)
中央级公益性科研院所基本科研业务费专项(1610012017002_05103)。
关键词
厌氧微生物
分离培养
高通量筛选
定向分离
anaerobes
isolation and cultivation
high-throughput screening
targeted isolation
