摘要
为探究绵羊肺炎支原体(Mo)致不同品种羊感染及病原核酸载量的差异,本研究利用Mo贵州威宁(GZ-WN)株分别感染5个品种羊,感染后死亡或迫杀后观察各组织的剖检病变,经荧光定量PCR检测各组织Mo的核酸载量,统计分析相同品种羊不同组织中Mo的核酸载量及不同品种羊相同组织中的核酸载量。结果显示,Mo可引起5个品种羊感染,死亡率呈现白山羊、黑山羊>麻羊>波尔山羊>湖羊;Mo感染可引起5个品种羊的肺脏、气管和胸腔出现不同程度的剖检病变,黑山羊肺脏粘连与白山羊气管黏液病变较其他品种羊严重;白山羊胸腔积液较其它品种羊严重,黑山羊次之;5个品种羊的心脏、肝脏、脾脏及肾脏均无肉眼可见病变。相同品种羊不同组织中Mo核酸载量的统计分析结果显示,相同品种羊的不同组织Mo核酸载量不同,各品种羊气管中的Mo核酸载量相对最高,极显著高于其他组织的核酸载量(p<0.01);其次是肺脏,而脾脏和肾脏中的Mo载量较低。不同品种羊相同组织中的Mo核酸载量统计结果显示,5个品种羊气管中Mo核酸载量最多的是波尔山羊,最少的是白山羊,且极显著低于其他品种羊(p<0.01);黑山羊肺脏中Mo核酸载量显著高于其他品种羊(p<0.05),湖羊Mo载量最少;心脏组织中,黑山羊Mo的核酸载量显著高于除麻羊以外的其他品种羊(p<0.05),载量最少的是白山羊;肝脏组织中,Mo核酸载量最多的是麻羊,显著高于除湖羊以外的其他品种羊(p<0.05),而白山羊最少;脾脏组织中,Mo核酸载量最多的是湖羊,显著高于其他品种羊(p<0.05),最少的是麻羊。肾脏组织中,Mo核酸载量最多的是湖羊,显著高于除波尔山羊外的其他品种羊(p<0.05),最少的是麻羊。上述结果表明,Mo感染相同品种羊存在组织病变和组织核酸载量的差异,且感染羊脏器组织Mo的核酸载量与组织病变程度和临床表现呈正相关;不同品种羊感染Mo后相同组织�
In order to e explore the infection outcomes and pathogen nucleic acid loads in different breeds of sheep infected with Mycoplasma pneumoniae(Mo),5 sheep breeds were infected with Mo Guizhou Weining(GZ-WN)strain.The pathological changes of each tissue were observed after infection or sacrificing,and the nucleic acid load of Mo in each tissue was detected by fluorescence quantitative PCR.The nucleic acid load of Mo in different tissues of the same breed of sheep and the nucleic acid load of the same tissue of different breeds of sheep were statistically analyzed.The results showed that Mo could cause infection in five breeds of sheep,and the mortality rate ranks as follows:white goat≈black goat>Ma sheep>Boer goat>Hu sheep.Mo infection could cause pathological changes of different degrees in lung,trachea and thorax of five breeds of sheep;lung adhesion and tracheal mucus lesion of black goat was more serious than that of other breeds;pleural effusion of white goat was more serious than that of other breeds,followed by black sheep.The heart,liver,spleen and kidney of the five breeds of sheep were not visible to the naked eye.The results of statistical analysis of Mo nucleic acid load in different tissues of the same sheep breed showed that the Mo nucleic acid load was different in different tissues of the same breed,and the Mo nucleic acid load in the trachea of each breed was the highest,which was significantly higher than that in other tissues(p<0.01),followed by lung,while Mo load in spleen and kidney was lower.The statistical results of Mo nucleic acid load in the same tissue of different breeds of sheep showed that the Mo nucleic acid load in the trachea of the five breeds was the highest in Boer goat and the least in white goat,which was significantly lower than that in other breeds(p<0.01);the Mo nucleic acid load in lung of black goat was significantly higher than that of other breeds(p<0.05);and the Mo load of Hu sheep was the lowest.In heart tissue,the nucleic acid load of Mo in black goat was significan
作者
陈静
王军
岳筠
张双翔
谢晓东
李涛
文明
程振涛
CHEN Jing;WANG Jun;YUE Jun;ZHANG Shuang-xiang;XIE Xiao-dong;LI Tao;WEN Ming;CHENG Zhen-tao(College of Animal Science,Guizhou University,Guiyang 550025,China;Key Laboratory of Animal Diseases and Veterinary Public Health in Guizhou Province,Guiyang 550025,China;Animal Disease Prevention and Control Center in Guizhou Province,Guiyang 550008,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2021年第2期128-133,共6页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金项目(31660723)
贵州省科技计划项目(黔科合基础[2019]1181号)
贵州省科技计划项目(黔科合支撑[2018]2271号)
贵州省科技计划项目(黔科合平台人才[2018]5253号)。
关键词
绵羊肺炎支原体
不同品种
羊
核酸载量
差异性分析
Mycoplasma pneumoniae
different breeds
sheep
nucleic acid load
difference analysis
