摘要
为了探究海拔高度对人类肠道菌群的影响,选取海拔3650~4500 m地区的67名藏族人,对其肠道菌群进行16S rRNA基因V3~V4区测序.对测序数据进行物种组成、alpha多样性、beta多样性、Pearson相关性、LEfSe、微生物网络、基因功能预测和肠型等生物信息学分析.研究结果表明:藏族人群肠道菌群的alpha多样性随海拔高度增加而降低,而beta多样性却与海拔高度的相关性较低,不同海拔高度人群的肠道菌群结构相似.LEfSe分析证明海拔3650 m藏族人群肠道菌群的biomarker为脱硫弧菌属;而海拔4000 m藏族人群肠道菌群的biomarker为梭菌属.Pearson相关性分析发现广古菌门、脱硫弧菌属、甲烷杆菌属、艰难杆菌属的丰度随着海拔高度的增加而显著下降.随着海拔高度的增加,藏族人群的肠道菌群微生物网络更加脆弱.总之,海拔高度是影响藏族人群肠道菌群alpha多样性的重要因素,不同海拔高度人群的肠道菌群差异显著.
In order to explore the impact of altitude on the intestinal microbiota,67 Tibetan stool samples from individuals who lived in the high-altitude environment of 3650-4500 m were selected,and 16S rRNA gene V3-V4 region sequencing of intestinal microbiota was performed.Bioinformatics analyses including community composition,alpha diversity,beta diversity,Pearson′s correlation,LEfSe,microbial network,gene function prediction and intestinal type were performed on sequencing data.It was found that the alpha diversity of intestinal microbiota of Tibetans decreased with the increase of altitude,while the beta diversity had a lower correlation with altitude.The structures of intestinal microbiota of Tibetans at different altitudes were similar.LEfSe analysis showed that the biomarker of the intestinal microbiota of Tibetans at an altitude of 3650 m was Desulfovibrio;while the biomarker of the intestinal microbiota of Tibetans at an altitude of 4000 m was Clostridium.Pearson correlation analysis showed that the relative abundance of Euryarchaeota,Desulfovibrio,Methanobrevibacter and Mogibacterium decreased significantly with the increase of altitude.The microbial network of the intestinal flora of the Tibetan population was more vulnerable with altitude.Overall,the research revealed differences in the intestinal microbiota among Tibetans living at different altituded and indicated that altitude was an essential factor affecting the alpha diversity of intestinal microbiota of Tibetans.
作者
梁田
马利锋
张致英
刘丽军
刘芳
董文学
李靖
张寒
蒋雅琼
白骕
康龙丽
LIANG Tian;MA Lifeng;ZHANG Zhiying;LIU Lijun;LIU Fang;DONG Wenxue;LI Jing;ZHANG Han;JIANG Yaqiong;BAI Su;KANG Longli(Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region,Xizang Minzu University,Xianyang 712082,Shaanxi Province,China;Key Laboratory of High Altitude Environment and Genes Related to Disease of Tibet Autonomous Region,Xizang Minzu University,Xianyang 712082,Shaanxi Province,China;Zhaxi Community Health Service Center,Chengguan District,Lhasa 850000,China)
出处
《天津师范大学学报（自然科学版）》
CAS
北大核心
2021年第2期36-43,共8页
Journal of Tianjin Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(31660307)
西藏自治区科技厅项目(XZ201801-GB-03)
西藏自治区自然科学基金资助项目(XZ2018ZRG-75(Z),XZ2019ZRG-133).
