摘要
目的:探讨藤茶中的主要活性成分二氢杨梅素(DMY)和杨梅苷(MYT)抑制HepG2细胞增殖的协同作用。方法:通过对体外培养的细胞分别给予DMY、MYT和DMY:MYT为1:4、1:2、1:1、2:1、4:1、8:1的混合物,采用MTT法和荧光染色法测定其对HepG2细胞活力的影响,并用合用指数(CI)和等效曲线法分析二者的协同作用。结果:DMY和MYT能够抑制HepG2肿瘤细胞的增殖,且IC50分别为56.46和95.01μmol/L。当二者比例为1:4~8:1时,对HepG2肿瘤细胞增殖的IC50为40.90~73.00μmol/L,CI值为0.60~0.86,其中1:1时协同作用最强,等效线法也表明二者存在协同作用。结论:DMY和MYT联用能够有效提升单体化合物对HepG2细胞的抑制作用,呈现出协同作用,这为藤茶进一步的功能研究和应用提供了依据。
Objective:To investigate the synergistic anti-tumor effect of Dihydromyricetin(DMY)and Myricitrin(MYT),which were the main active compounds in vine tea.Methods:The HepG2 cells were treated with DMY,MYT and DMY:MYT at the ratios of 1:4,1:2,1:1,2:1,4:1,8:1,respectively.The activity of HepG2 cell was measured by MTT assay and fluorescence staining.The synergistic effect of DMY and MYT was analyzed by combination index(CI)and isobole.Results:DMY and MYT could inhibit the proliferation of HepG2 cells with IC50 values of 56.46 and 95.01μmol/L,respectively.When the mixed ratios of the two compounds were 1:4~8:1,their IC50 values on the HepG2 cell proliferation were 40.90~73.00μmol/L,and the CI values were 0.60~0.86.The mixed ratio of 1:1 showed the strongest synergistic effect,isobole analysis also indicated that there was a synergistic effect between two compounds.Conclusions:The combination of DMY and MYT could effectively promote the activity on inhibiting HepG2 cell proliferation,showing a synergistic effect,which would provide a basis for the further functional research and application of vine tea.
作者
陈秋平
林心健
李申兰
刘合生
戚向阳
CHEN Qiuping;LIN Xinjian;LI Shenlan;LIU Hesheng;QI Xiangyang(College of Biological&Environmental Sciences,Zhejang Wanli University,Ningbo 315100,China)
出处
《食品工业科技》
CAS
北大核心
2021年第8期333-337,共5页
Science and Technology of Food Industry
基金
浙江省"生物工程"一流学科自设课题(ZS2019005)
宁波市高校“食品科学与工程”重点学科自设课题(2019FSEKDNB003)。
