摘要
目的检测人多梳蛋白2(HPC2)和Ki67在鼻咽癌组织中的表达,探讨其表达与临床病理因素的关系,揭示两者表达相关性及其预后意义。方法采用Western blot法检测HPC2在鼻咽癌及非肿瘤鼻咽上皮组织中的表达,进一步选取126例鼻咽癌活检石蜡组织标本,免疫组化法检测HPC2和Ki67的表达情况,结合临床病理资料及随访数据统计分析。结果HPC2在鼻咽癌组织的表达较鼻咽非肿瘤组织的表达上调,HPC2和Ki67的蛋白表达均定位于鼻咽癌组织细胞核,HPC2高表达占55.6%,Ki67高表达占61.9%。HPC2的表达与鼻咽癌的TNM分期、复发、转移有关。HPC2和Ki67的表达呈正相关(r=0.354,P<0.01)。HPC2和Ki67联合检测在NPC的无进展生存(PFS)和总生存(OS)的预后风险模型显示,两者均高表达者为高风险组,预后最差,两者表达不一致为中度风险,预后中等;如果两者均低表达为低度风险,预后最好(P=0.000)。结论HPC2高表达状态与鼻咽癌临床进展及预后不良有关,联合Ki67检测的预后模型有利于更准确地评估预后。
Objective To detect the expression of HPC2 and Ki67 in nasopharyngeal carcinoma,to explore the relationship between the expression and clinicopathological factors,and to reveal the correlation between their expression and prognostic significance.Methods The expression of HPC2 in nasopharyngeal carcinoma and non-tumor nasopharyngeal epithelium was detected by Western blot.Furthermore,the expression of HPC2 and Ki67 were detected in 126 NPC biopsies by immunohistochemistry.The clinicopathological data and follow-up data were analyzed.Results The protein expression of HPC2 and Ki67 was localized in the nucleus of NPC.High expression of HPC2 accounted for 55.6%,and high expression of Ki67 accounted for 61.9%.The expression of HPC2 was related to TNM stage,recurrence and expression of Ki67.There was a positive correlation between the expression of HPC2 and Ki67(r=0.354,P<0.01).The prognostic risk models of progression-free survival(PFS)and overall survival(OS)of NPC detected jointly by HPC2 and Ki67 showed that those with high expression of both were at high risk and had the worst outcome,while those with inconsistent expression were at moderate risk and had moderate outcome.The outcome was best if both were low expression at low risk(P=0.000).Conclusion The high expression of HPC2 was related to the clinical progress and worse outcome of NPC,which combined with the detection of Ki67 was valuable to evaluate the prognosis more accurately.
作者
吴梅
侯小娟
再努拉·艾未肉拉
郭小平
唐亮
Wu Mei;Hou Xiaojuan;Zainula·Aiweiroula(People′s Hospital Otolaryngology Diagnosis and Treatment Center,Xinjiang Uygur Autonomous Region,Xinjiang 830001,China)
出处
《医学研究杂志》
2021年第4期46-50,共5页
Journal of Medical Research
基金
新疆维吾尔自治区自然科学基金资助项目(2017D01C093)。
