摘要
目的 分析子宫内膜异位症(endometriosis,EM)患者生物信息,探索异位内膜(ectopic endo-metrium,EC)组织中异常表达基因.方法 获取EM患者组织测序数据,筛选差异表达基因(differentially expressed genes,DEGs),使用DAVID工具进行DEGs基因注释,使用STRING在线分析DEGs编码蛋白间相互作用(protein protein interaction,PPI),确定候选基因.招募30名EM患者采集EC组织与在位内膜(EU)组织,验证候选基因表达.结果 筛选出826个DEGs,低表达665个,高表达161个.PPI分析确定13个候选基因.实时荧光定量PCR(real-time fluorescent quantitative PCR,qRT-PCR)验证,实验组HOXC4、HOXC6表达显著低于对照组(P<0.01),实验组HOXA5、HOXB7显著高于对照组(P<0.01).结论 HOX基因可能直接参与EM进展,检测HOX基因有望为揭示EM生物学机制提供依据.
Objective To explore the gene changes in patients with endometriosis(EM)by analyzing the bioinformatics data.Methods High-throughput sequencing data was gained from data sets.DEGs were identified by screening condition.The online DAVID tool was used for gene annotation.Protein protein interaction(PPI)analysis was performed using STRING online tool,and hub genes were screened out by STRING analysis.qRT-PCR was carried out to validate the expression level of candidate genes in 30 ectopic endometrium(EC)and eutopic endometrium(EU)tissue from patients with EM.Results A total of 826 DEGs were obtained,including 161 up-regulated genes and 665 down-regulated genes.13 genes were screened out as candidate genes by PPI analysis.By analysis of qRT-PCR,HOXC4,HOXC6 were validated low expression in test group(P<0.01),HOXA5,HOXB7 was validated high expression in test group(P<0.01).Conclusion HOX genes may be directly involved in EM development.The detection of HOX genes is expected to provide scientific basis for revealing the biological mechanism of EM.
作者
穆艳超
骈朋云
司雯
MU Yanchao;PIAN Pengyun;SI Wen(Laboratory Medicine,Anyang Maternity and Child Healthcare Hospital,Anyang,Henan,455000,China)
出处
《医学分子生物学杂志》
CAS
2021年第2期153-156,共4页
Journal of Medical Molecular Biology
