摘要
目的探讨苦参碱通过IL-6/STAT3信号通路抑制人结肠癌细胞增殖、转移的机制研究。方法通过使用苦参碱干预人结肠癌细胞HCT-116,使用细胞增殖实验CCK-8检测细胞增殖力,使用Western blot实验检测炎性因子IL-6/STAT3基因蛋白表达水平以及结肠癌细胞转移力E-Cad基因蛋白表达水平。结果细胞增殖试验显示,2mg/m L、3mg/m L、4mg/m L苦参碱组较对照组细胞增殖力明显降低。Western blot结果显示,苦参碱组较对照组IL-6/STAT3蛋白表达量明显降低(P<0.05),E-Cad蛋白表达量明显升高(P<0.05)。结论苦参碱可通过抑制IL-6/STAT3信号通路降低人结肠癌细胞HCT-116炎性反应,进一步抑制人结肠癌细胞HCT-116的增殖和转移力,从而达到抗肿瘤作用。
Objective To study the mechanism of matrine inhibiting human colon cancer cells through the IL-6/STAT3 signaling pathway.Method Human colon cancer cell HCT-116 was interfered with matrine,cell proliferation assay CCK-8 was used to detect cell proliferation,and the protein expression level of inflammatory factor IL-6/STAT3 gene and the protein expression level of metastatic power of colon cancer cell E-CAD gene were detected by Western blot assay.Results The cell proliferation test showed that the cell proliferation of 2 mg/mL,3 mg/mL and 4 mg/mL matrine groups was significantly lower than that of the control group.Western blot results showed that the matrine group had IL-6/STAT3 protein compared with the control The expression level was significantly decreased(P<0.05),and the expression level of E-Cad protein was significantly increased(P<0.05).Conclusion Matrine can reduce the inflammatory response of human colon cancer cell HCT-116 by inhibiting IL-6/STAT3 signaling pathway,and further inhibit the proliferation and invasiveness of human colon cancer cell HCT-116,so as to achieve anti-tumor effect.
作者
刘晔
刘斌
LIU Ye;LIU Bin(Department of Laboratory Medicine,Jingbian County People's Hospital,Jingbian Shanxi,718500,China;Department of Laboratory Medicine,Jingbian County Hospital of Traditional Chinese Medicine)
出处
《承德医学院学报》
2021年第2期100-102,共3页
Journal of Chengde Medical University
