摘要
目的探讨外周血LTA4H基因多态性rs7971150位点检测在老年慢性阻塞性肺疾病(COPD)患者中的临床意义以及与吸烟的关系。方法选择2017年9月-2020年1月在上海市浦东新区浦南医院和上海杨思医院呼吸科病房住院的150例COPD急性加重患者为观察组,150例支气管扩张急性加重和社区获得性肺炎患者为对照组,2组患者根据是否吸烟分为不吸烟组和吸烟组2个亚组,采用imLDR多重连接酶SNP分型技术,检测患者外周血LTA4H基因多态性rs7971150,并检测动脉血气(pH值、动脉血氧分压、动脉血二氧化碳分压)、肺功能指标(FEV1、FEV1%)、6分钟步行距离。结果 2组rs7971150位点基因型频率分布的差异有统计学意义(P<0.05),与AA基因型相比较,GG基因型显著增加了患COPD风险(P<0.05)。在吸烟亚组和不吸烟亚组中,rs7971150位点基因型频率在观察组与对照组之间分布的差异均有统计学意义(P<0.05),且在吸烟亚组中,与AA基因型相比较,GA、GG基因型显著增加了患COPD风险(P<0.05)。在观察组中,不吸烟患者与吸烟患者的pH值、PaO2、PaCO2之间的差异均无统计学意义(P均>0.05)而FEV1、FEV1%、6分钟步行距离之间的差异均有统计学意义(P均<0.05)。对照组与观察组pH值、PaO2之间的差异均无统计学意义(P均>0.05),而PaCO2、FEV1、FEV1%、6分钟步行距离之间的差异均有统计学意义(P均<0.05)。结论 LTA4H基因多态性rs7971150位点测定显示,GA、GG基因型显著增加了 COPD患病风险。与不吸烟的COPD患者相比,吸烟的COPD患者存在明显的GA、GG突变基因型,以及较差的肺功能、较短的6分钟步行距离。
Objective To investigate the clinical significance of detection of LTA4H rs7971150 gene polymorphism in peripheral blood of elderly patients with chronic obstructive pulmonary disease(COPD)and its relationship with smoking.Methods From September 2017 to January 2020,150 patients with acute exacerbation of COPD who were hospitalized in the Department of Respiratory Disease of Yangsi Hospital and Department of Respiratory Disease of Pu'nan Hospital of Pudong New District were selected as the observation group,and 150 patients with acute exacerbation of bronchiectasis and community-acquired pneumonia were selected as the control group.The patients of both groups were further divided into two subgroups,respectively:non-smoking group and smoking group according to whether they smoked or not.imLDR multi-ligase SNP typing technology was used to detect LTA4H rs7971150 gene polymorphisms in peripheral blood of patients,and the arterial blood gases indexes(pH,arterial oxygen partial pressure,arterial carbon dioxide partial pressure),lung function indexes(FEV,.FEV%),6 minutes walking distance(6MWD)were compared between the two groups.Results The difference in frequency distribution of rs7971150 locus between the two groups was statistically significant P<0.05).Compared with AA genotype,GG genotype significantly increased the risk of COPD(P<0.05).In the smoking and non smoking subgroups,there were statistically significant differences in the genotype frequency of rs7971150 between the observation group and the control group(P<0.05).In the smoking subgroup,GA and GG genotypes significantly increased the risk of COPD compared with AA genotypes(P<0.05).In the observation group,there were no significant differences in pH,PaO_(2)and PaCO_(2)between nonsmoking subgroup and smoking subgroup(allP>0.05),while there were significant differences in FEV1,FEV1%and 6MWD between non-smoking subgroups and smoking subgroup(all P<0.05).There were no significant differences in pH and PaO_(2)between the control group and the observation group(P
作者
程炜炜
鲁晓怡
周晓莉
刘明
张青
夏娟
赵云峰
Cheng Weiwei;Lu Xiaoyi;Zhou Xiaoli;Liu Ming;Zhang Qing;Xia Juan;ZhaoYunfeng(Department of Respiratory Disease,Yangsi Hospital,Shanghai,200126,P.R.China;Department of Respiratory Disease,Pu'nan Hospital,Pudong New District,Shanghai,200125,P.R.China)
出处
《老年医学与保健》
CAS
2021年第2期266-269,共4页
Geriatrics & Health Care
基金
上海市浦东新区卫生系统重点专科培养计划(PWZzk2017-18)
上海市浦东新区卫生系统领先人才培养计划(PWR12017-01)
