摘要
建立超高效液相色谱-串联质谱法测定人血清中氯氮平、氟哌啶醇、氟奋乃静、丙咪嗪4种镇静催眠类药物的方法。样品经乙腈提取,采用ACQUITY UPLC HSS T3色谱柱分离,以乙腈-水溶液(含0.05%甲酸)为流动相梯度洗脱,流量为0.3 mL/min,进样体积为10μL,多反应监测模式检测。4种镇静催眠类药物在各自的质量浓度范围内与色谱峰面积具有良好的线性关系,相关系数均大于0.995,氯氮平、氟哌啶醇、丙咪嗪的检出限均为0.03μg/L,氟奋乃静的检出限为0.06μg/L。测定结果的相对标准偏差为1.1%〜8.9%(n=6),加标样品平均回收率为90.7%〜111.9%。该方法准确、可靠,适用于人血清中镇静催眠类药物的检测。
A method for the determination of clozapine,haloperidol,fluphenazine and imipramine in human serum by ultra performance liquid chromatography-tandem mass spectrometry was established.The sample was extracted with acetonitrile and separated on acquity UPLC HSS T3 column.The mobile phase was acetonitrile water solution(containing 0.05%formic acid)with gradient elution.The flow rate was 0.3 mL/min and the injection volume was 10μL.The multi reaction monitoring mode was used for detection.There were good linear relationships between the four sedative hypnotic drugs concentration and the chromatographic peak area in their respective concentration range,and the correlation coefficients were all more than 0.995.The detection limits of clozapine,haloperidol and imipramine were 0.03μg/L,and the detection limit of fluphenazine was 0.06μg/L.The relative standard deviation of the results were 1.1%-8.9%(n=6),and the average recoveries of spiked samples were 90.7%-111.9%.The method is accurate,reliable and suitable for the determination of sedative and hypnotic drugs in human serum.
作者
于浩洋
李颜岩
冯静
华正罡
Yu Haoyang;LI Yanyan;Feng Jing;Hua Zhenggang(Liaoning Center for Disease Control and Prevention,Shenyang 110005,China)
出处
《化学分析计量》
CAS
2021年第4期38-41,共4页
Chemical Analysis And Meterage
关键词
超高效液相色谱-串联质谱法
人血清
镇静催眠类药物
ultra high performance liquid chromatography-tandem mass spectrometry
human serum
sedative hypnotic drugs
