摘要
目的:探讨胸腺基质淋巴细胞生成素(Thymic stromal lymphopoietin,TSLP)在脓毒症患者血清中的表达及临床意义。方法:收集2017年1月-2019年10月在本院ICU住院治疗的患者80例为研究对象,共分三组,对照组(C组,n=30),脓毒症组(S组,n=30),脓毒症休克组(SS组,n=20)。采集所有研究对象入ICU后24 h内的临床资料、检查结果及空腹静脉血5 mL,用双抗体夹心酶联免疫吸附法(ELISA)测定血清TSLP,IL-6的浓度,分析两者在脓毒症患者中的表达及相关性。结果:与C组患者相比,S组及SS组患者在性别构成、年龄、APACHEⅡ评分、白细胞计数上差异均无统计学意义(P>0.05);S组患者血清PCT浓度与C组比较,差异无统计学意义(P>0.05);SS组患者血清PCT浓度显著高于C组及S组,差异均有统计学意义(P<0.0001)。S组患者血清TSLP的浓度显著高于C组(P<0.0001),IL-6的浓度高于C组(P=0.030)。SS组患者血清TSLP高于S组(P=0.010),IL-6的浓度显著高于S组(P<0.0001)。S组患者血清中TSLP的浓度与IL-6的浓度呈明显正相关(r=0.61,P<0.01)。结论:TSLP在脓毒症患者血清中表达增高,且与脓毒症的严重程度相关,提示TSLP参与脓毒症的发病机制。
Objective:To investigate the expression and clinical significance of thymic stromal Lymphopoietin(TSLP)in serum of patients with sepsis.Method:Eighty patients admitted to ICU of our hospital from January 2017 to October 2019 were selected and divided into three groups:control group(group C,n=30),septic group(group S,n=30)and septic shock group(group SS,n=20).Clinical and examination data and 5 mL venous blood of the patients were collected within 24 hours after be admitted to ICU.The serum levels of TSLP and IL-6 were measured by double antibody sandwich enzyme linked immunosorbent assay(ELISA).The expressions and correlation of TSLP and IL-6 in septic patients were analyzed.Result:Compared with group C,there were no significant differences in gender composition,age,APACHEⅡscore and white blood cell count between group S and group SS(P>0.05);there was no significant difference in serum PCT concentration between group S and group C(P>0.05);the serum PCT concentration in group SS was significantly higher than that in group C and group S(P<0.0001).The concentration of serum TSLP in group S was significantly higher than that in group C(P<0.0001),and the concentration of IL-6 in group S was higher than that in group C(P=0.030).The serum TSLP of group SS was higher than that of group S(P=0.010),and the concentration of IL-6 was significantly higher than that of group S(P<0.0001).There was a positive correlation between TSLP and IL-6 in group S(r=0.61,P<0.01).Conclusion:The expression of TSLP in the serum of patients with sepsis is increased and related to the severity of sepsis,which suggests that TSLP may be involved in the pathogenesis of sepsis.
作者
陈晶莹
罗惠君
何文成
张卫星
罗华
张声
CHEN Jingying;LUO Huijun;HE Wencheng;ZHANG Weixing;LUO Hua;ZHANG Sheng(Peking University Shenzhen Hospital,Shenzhen 518035,China;不详)
出处
《中国医学创新》
CAS
2021年第7期114-118,共5页
Medical Innovation of China
基金
深圳市卫生健康委员会学科建设能力提升项目(201606006)。
