摘要
目的运用网络药理学方法研究桑黄治疗肝炎的有效成分及作用靶点,为深入探索其药理作用机制提供依据。方法本研究采用TCMSP平台中的CancerHSP软件检索桑黄的化学成分、作用靶点,通过Uniprot数据库及GendCards数据库分别检索各化学成分和肝炎对应的靶基因,找出关键基因;采用Cytoscape 3.7.2软件做出桑黄-肝炎-靶点网络图;通过String数据库构建蛋白质相互作用(PPI)网络图;利用ClueGo软件对关键靶点进行功能富集分析及KEGG通路分析。结果经检索桑黄共有9种化合物,经筛选得到3个化合物、27个靶点、30个基因,与肝炎相关的有MAOB、XDH、ALDH2、SMAD3、ALDH3A1、CYP2A6等22个关键基因。主要通过组氨酸代谢(histidine metabolism)、酪氨酸代谢(tyrosine metabolism)等7条通路发挥作用。结论本研究初步验证了桑黄治疗肝炎的作用靶点及作用通路,为进一步揭示其肝脏保护机制奠定基础。
Objective To study the active components and action targets of Phellinus igniarius in the treatment of hepatitis by network pharmacology.So as to provide a basis for further exploration of its pharmacological action mechanism.Methods This study used the CancerHSP in the TCMSP platform to retrieve the chemical constituents and action targets of Phellinus igniarius.Cytoscape 3.7.2 was used to make the network map of Phellinus igniarius-Hepatitis-target.Target protein-protein interaction(PPI)network was constructed by using String database.ClueGo software was used to perform functional enrichment analysis and KEGG pathway analysis of key targets.Results A total of 9 compounds were retrieved and 3 compounds,27 targets and 30 genes were screened out.There are 22 key genes associated with hepatitis,including MAOB,XDH,ALDH2,SMAD3,ALDH3A1 and CYP2A6.Histidine metabolism,Tyrosine metabolism and other 7 pathways play a role.Conclusion This study preliminarily verified the therapeutic target and pathway of Phellinus igniarius in the treatment of hepatitis,and laid a foundation for further revealing its liver protection mechanism.
作者
丁静
吕海芹
王鹤飞
张晗
陈文文
党和勤
刘燕琳
DING Jing;LYU Haiqin;WANG Hefei;ZHANG Han;CHEN Wenwen;DANG Heqin;LIU Yanlin(Department of Pharmacy,The Second Affiliated Hospital of Shandong First Medical University,Tai′an 271000,China;Department of Pharmacy,Shandong Province Coal Taishan Sanatorium,Tai′an 271000,China)
出处
《药学研究》
CAS
2021年第3期144-148,共5页
Journal of Pharmaceutical Research
基金
山东第一医科大学潜力提升计划(No.2019QL017)
山东省医药卫生科技发展计划(No.2018WS120)
山东省中医药科技发展计划项目(No.2015-264)
泰山医学院附属医院青年基金(No.2014QN05)。
关键词
桑黄
肝炎
网络药理学
作用机制
Phellinus igniarius
Hepatitis
Network pharmacology
Mechanism
